diff --git a/sdk/monitor/azure-monitor-opentelemetry/CHANGELOG.md b/sdk/monitor/azure-monitor-opentelemetry/CHANGELOG.md
index 8e98f6de937f..2eaaeec33b77 100644
--- a/sdk/monitor/azure-monitor-opentelemetry/CHANGELOG.md
+++ b/sdk/monitor/azure-monitor-opentelemetry/CHANGELOG.md
@@ -10,6 +10,8 @@
     ([#31740](https://github.com/Azure/azure-sdk-for-python/pull/31740))
 - Un-vendoring instrumentations
     ([#31744](https://github.com/Azure/azure-sdk-for-python/pull/31740))
+- Add preview warning for Autoinstrumentation entry points
+    ([#31767](https://github.com/Azure/azure-sdk-for-python/pull/31767))
 
 ### Breaking Changes
 
diff --git a/sdk/monitor/azure-monitor-opentelemetry/azure/monitor/opentelemetry/_constants.py b/sdk/monitor/azure-monitor-opentelemetry/azure/monitor/opentelemetry/_constants.py
index 98ebb0c26873..079ce1f9c024 100644
--- a/sdk/monitor/azure-monitor-opentelemetry/azure/monitor/opentelemetry/_constants.py
+++ b/sdk/monitor/azure-monitor-opentelemetry/azure/monitor/opentelemetry/_constants.py
@@ -7,6 +7,7 @@
 import logging
 import platform
 from os import environ
+from os.path import isdir
 from pathlib import Path
 
 from azure.monitor.opentelemetry.exporter._connection_string_parser import ( # pylint: disable=import-error
@@ -36,6 +37,7 @@
 #     "AZURE_MONITOR_OPENTELEMETRY_DISTRO_ENABLE_EXPORTER_DIAGNOSTICS"
 # )
 _CUSTOMER_IKEY_ENV_VAR = None
+_PREVIEW_ENTRY_POINT_WARNING = "Autoinstrumentation for the Azure Monitor OpenTelemetry Distro is in preview."
 logger = logging.getLogger(__name__)
 
 
@@ -85,3 +87,7 @@ def _env_var_or_default(var_name, default_val=""):
 )
 # TODO: Enabled when duplicate logging issue is solved
 # _EXPORTER_DIAGNOSTICS_ENABLED = _is_exporter_diagnostics_enabled()
+
+
+def _is_attach_enabled():
+    return isdir("/agents/python/")
diff --git a/sdk/monitor/azure-monitor-opentelemetry/azure/monitor/opentelemetry/autoinstrumentation/_configurator.py b/sdk/monitor/azure-monitor-opentelemetry/azure/monitor/opentelemetry/autoinstrumentation/_configurator.py
index 8988cb1ab8d8..c3bedbae6504 100644
--- a/sdk/monitor/azure-monitor-opentelemetry/azure/monitor/opentelemetry/autoinstrumentation/_configurator.py
+++ b/sdk/monitor/azure-monitor-opentelemetry/azure/monitor/opentelemetry/autoinstrumentation/_configurator.py
@@ -6,9 +6,14 @@
 
 
 import logging
+from warnings import warn
 
 from opentelemetry.sdk._configuration import _OTelSDKConfigurator
 
+from azure.monitor.opentelemetry._constants import (
+    _is_attach_enabled,
+    _PREVIEW_ENTRY_POINT_WARNING,
+)
 from azure.monitor.opentelemetry.diagnostics._diagnostic_logging import (
     AzureDiagnosticLogging,
 )
@@ -18,6 +23,8 @@
 
 class AzureMonitorConfigurator(_OTelSDKConfigurator):
     def _configure(self, **kwargs):
+        if not _is_attach_enabled():
+            warn(_PREVIEW_ENTRY_POINT_WARNING)
         try:
             AzureDiagnosticLogging.enable(_logger)
             super()._configure(**kwargs)
diff --git a/sdk/monitor/azure-monitor-opentelemetry/azure/monitor/opentelemetry/autoinstrumentation/_distro.py b/sdk/monitor/azure-monitor-opentelemetry/azure/monitor/opentelemetry/autoinstrumentation/_distro.py
index 2911421ef8dd..e9c13f77f9da 100644
--- a/sdk/monitor/azure-monitor-opentelemetry/azure/monitor/opentelemetry/autoinstrumentation/_distro.py
+++ b/sdk/monitor/azure-monitor-opentelemetry/azure/monitor/opentelemetry/autoinstrumentation/_distro.py
@@ -5,6 +5,7 @@
 # --------------------------------------------------------------------------
 import logging
 from os import environ
+from warnings import warn
 
 from opentelemetry.environment_variables import (
     OTEL_LOGS_EXPORTER,
@@ -20,6 +21,10 @@
 
 from azure.core.settings import settings
 from azure.core.tracing.ext.opentelemetry_span import OpenTelemetrySpan
+from azure.monitor.opentelemetry._constants import (
+    _is_attach_enabled,
+    _PREVIEW_ENTRY_POINT_WARNING,
+)
 from azure.monitor.opentelemetry.diagnostics._diagnostic_logging import (
     AzureDiagnosticLogging,
 )
@@ -37,6 +42,8 @@
 
 class AzureMonitorDistro(BaseDistro):
     def _configure(self, **kwargs) -> None:
+        if not _is_attach_enabled():
+            warn(_PREVIEW_ENTRY_POINT_WARNING)
         try:
             _configure_auto_instrumentation()
         except Exception as ex:
diff --git a/sdk/monitor/azure-monitor-opentelemetry/tests/autoinstrumentation/test_configurator.py b/sdk/monitor/azure-monitor-opentelemetry/tests/autoinstrumentation/test_configurator.py
new file mode 100644
index 000000000000..72897c09d918
--- /dev/null
+++ b/sdk/monitor/azure-monitor-opentelemetry/tests/autoinstrumentation/test_configurator.py
@@ -0,0 +1,31 @@
+import warnings
+from unittest import TestCase
+from unittest.mock import patch
+
+from azure.core.tracing.ext.opentelemetry_span import OpenTelemetrySpan
+from azure.monitor.opentelemetry.autoinstrumentation._configurator import (
+    AzureMonitorConfigurator,
+)
+
+
+class TestConfigurator(TestCase):
+    @patch("azure.monitor.opentelemetry.autoinstrumentation._configurator._is_attach_enabled", return_value=True)
+    @patch(
+        "azure.monitor.opentelemetry.autoinstrumentation._configurator.AzureDiagnosticLogging.enable"
+    )
+    def test_configure(self, mock_diagnostics, attach_mock):
+        configurator = AzureMonitorConfigurator()
+        with warnings.catch_warnings():
+            warnings.simplefilter("error")
+            configurator._configure()
+        mock_diagnostics.assert_called_once()
+
+    @patch("azure.monitor.opentelemetry.autoinstrumentation._configurator._is_attach_enabled", return_value=False)
+    @patch(
+        "azure.monitor.opentelemetry.autoinstrumentation._configurator.AzureDiagnosticLogging.enable"
+    )
+    def test_configure_preview(self, mock_diagnostics, attach_mock):
+        configurator = AzureMonitorConfigurator()
+        with self.assertWarns(Warning):
+            configurator._configure()
+        mock_diagnostics.assert_called_once()
diff --git a/sdk/monitor/azure-monitor-opentelemetry/tests/autoinstrumentation/test_distro.py b/sdk/monitor/azure-monitor-opentelemetry/tests/autoinstrumentation/test_distro.py
index f161506f0af7..1b91ae1de8c6 100644
--- a/sdk/monitor/azure-monitor-opentelemetry/tests/autoinstrumentation/test_distro.py
+++ b/sdk/monitor/azure-monitor-opentelemetry/tests/autoinstrumentation/test_distro.py
@@ -1,3 +1,4 @@
+import warnings
 from unittest import TestCase
 from unittest.mock import patch
 
@@ -8,13 +9,30 @@
 
 
 class TestDistro(TestCase):
+    @patch("azure.monitor.opentelemetry.autoinstrumentation._distro._is_attach_enabled", return_value=True)
     @patch("azure.monitor.opentelemetry.autoinstrumentation._distro.settings")
     @patch(
         "azure.monitor.opentelemetry.autoinstrumentation._distro.AzureDiagnosticLogging.enable"
     )
-    def test_configure(self, mock_diagnostics, azure_core_mock):
+    def test_configure(self, mock_diagnostics, azure_core_mock, attach_mock):
         distro = AzureMonitorDistro()
-        distro.configure()
+        with warnings.catch_warnings():
+            warnings.simplefilter("error")
+            distro.configure()
+        self.assertEqual(mock_diagnostics.call_count, 2)
+        self.assertEqual(
+            azure_core_mock.tracing_implementation, OpenTelemetrySpan
+        )
+
+    @patch("azure.monitor.opentelemetry.autoinstrumentation._distro._is_attach_enabled", return_value=False)
+    @patch("azure.monitor.opentelemetry.autoinstrumentation._distro.settings")
+    @patch(
+        "azure.monitor.opentelemetry.autoinstrumentation._distro.AzureDiagnosticLogging.enable"
+    )
+    def test_configure_preview(self, mock_diagnostics, azure_core_mock, attach_mock):
+        distro = AzureMonitorDistro()
+        with self.assertWarns(Warning):
+            distro.configure()
         self.assertEqual(mock_diagnostics.call_count, 2)
         self.assertEqual(
             azure_core_mock.tracing_implementation, OpenTelemetrySpan
diff --git a/sdk/monitor/azure-monitor-opentelemetry/tests/test_constants.py b/sdk/monitor/azure-monitor-opentelemetry/tests/test_constants.py
index adb7b7d0d648..b78f1846bf98 100644
--- a/sdk/monitor/azure-monitor-opentelemetry/tests/test_constants.py
+++ b/sdk/monitor/azure-monitor-opentelemetry/tests/test_constants.py
@@ -151,3 +151,21 @@ def test_env_var_or_default_empty_with_defaults(self):
         self.assertEqual(
             _constants._env_var_or_default("key", default_val="value"), "value"
         )
+
+    @patch(
+        "azure.monitor.opentelemetry._constants.isdir",
+        return_value=True,
+    )
+    def test_attach_enabled(self, mock_isdir):
+        self.assertEqual(
+            _constants._is_attach_enabled(), True
+        )
+
+    @patch(
+        "azure.monitor.opentelemetry._constants.isdir",
+        return_value=False,
+    )
+    def test_attach_disabled(self, mock_isdir):
+        self.assertEqual(
+            _constants._is_attach_enabled(), False
+        )