serprotev.html

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<!-- Page Title: Serine Protease Evolution -->
<h2>Introduction</h2>
<p>
    These scripts compare the substrate-binding pockets of elastase and
    chymotrypsin, two serine proteases. (Based on 7est.pdb, de la Sierra et al.
    (1990) J.Mol.Recog. <strong>3</strong>: 36-44)
</p>
<br>

<h2>Serine Protease Evolution 1</h2>
<p>
    <span style="color:blue;">Elastase</span> and <span style="color:lime;">
    chymotrypsin</span> are both serine proteases. Note the close similarity of
    the tertiary structures of these enzymes.
</p>
<!-- JSMol Window Container -->
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<!-- UI Control Box -->
<div style="text-align: center;">
    <!-- give the button a right margin for spacing -->
    <button type="button" id="resetBtn1" style="margin-right: 10px;">Reset</button>
    <input type="checkbox" id="spinBox1"> Spin On/Off
</div>
<br>

<h2>Serine Protease Evolution 2</h2>
<p>
    Indeed, the structure of the catalytic triad is almost identical in the two
    enzymes.
</p>
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<div style="text-align: center; margin-top: 10px;">
    <button type="button" id="resetBtn2" style="margin-right: 10px;">Reset</button>
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</div>
<br>

<h2>Serine Protease Evolution 3</h2>
<p>
    Elastase has a specificity for substrates with <span style="color:red;">
    small alipathic sidechains</span>, which bind near the
    <span style="color:lime;">catalytic triad</span> and are held in place by
    <span style="color:orange;">large bulky</span> sidechains of the enzyme.
</p>
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</div>
<br>

<h2>Serine Protease Evolution 4</h2>
<p>
    Chymotrypsin has a specificity for substrates with
    <span style="color:magenta;">large aromatic sidechains</span>, which bind
    near the <span style="color:lime;">catalytic triad</span> and are held in
    place by a binding pocket whose entrance contains Gly
    (<span style="color:gold;">YELLOW</span>) sidechains with
    <span style="color:blue;">Ser</span> at the bottom of the pocket.
</p>
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    <input type="checkbox" id="spinBox4"> Spin On/Off
</div>
<br>

<h2>Serine Protease Evolution 5</h2>
<p>
    Here we see how the large sidechain of the <span style="color:magenta;">
    chymotrypsin substrate</span> is incompatible with the
    <span style="color:orange;">elastase</span> binding pocket.
</p>
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</div>
<br>

<h2>Serine Protease Evolution 6</h2>
<p>
    Here we compare the binding of the <span style="color:magenta;">chymotrypsin
    substrate</span> to <span style="color:lime;">chymotrypsin</span> and
    <span style="color:orange;">elastase</span>.
</p>
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</div>
<br>

<h2>Serine Protease Evolution 7</h2>
<p>
    Here we compare the binding of the <span style="color:red;">elastase
    substrate</span> to <span style="color:lime;">chymotrypsin</span> and
    <span style="color:orange;">elastase</span>.
</p>
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