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Merge pull request #760 from FelixKrueger/dev
merging bclconvert fix
2 parents 0ad1701 + aebf069 commit 44618d2

13 files changed

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CHANGELOG.md

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# Bismark Changelog
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## Changelog for Bismark v0.25.0 (release on 03 Aug 2025)
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## Changelog for Bismark v0.25.1 (release on 06 Aug 2025)
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### deduplicate_bismark
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- Also allowing the `+` sign as valid symbol when considering UMIs in `--bclconvert` mode [commit](268a8a26997f847c8e404f55cbdfb753a044190c)
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## Changelog for Bismark v0.25.0 (release on 03 Aug 2025)
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### Bismark
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- now using 4 cores for merging multiple BAM files (more details [#707](https://github.com/FelixKrueger/Bismark/issues/707))

NOMe_filtering

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my %chromosomes; # storing sequence information of all chromosomes/scaffolds
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my %processed; # keeping a record of which chromosomes have been processed
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my $nome_version = 'v0.25.0';
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my $nome_version = 'v0.25.1';
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my ($output_dir,$genome_folder,$zero,$CpG_only,$CX_context,$split_by_chromosome,$parent_dir,$coverage_infile,$cytosine_out,$merge_CpGs,$gc_context,$gzip,$nome) = process_commandline();
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bam2nuc

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my %genomic_freqs;
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my %processed;
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my $bam2nuc_version = 'v0.25.0';
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my $bam2nuc_version = 'v0.25.1';
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my ($output_dir,$genome_folder,$parent_dir,$samtools_path,$genome_freq_only) = process_commandline();
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bismark

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my $parent_dir = getcwd();
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my $bismark_version = 'v0.25.0';
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my $bismark_version = 'v0.25.1';
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my $copyright_dates = "2010-25";
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my $start_run = time();

bismark2bedGraph

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## You should have received a copy of the GNU General Public License
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## along with this program. If not, see <http://www.gnu.org/licenses/>.
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my $bismark2bedGraph_version = 'v0.25.0';
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my $bismark2bedGraph_version = 'v0.25.1';
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my @bedfiles;
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my @methylcalls = qw (0 0 0); # [0] = methylated, [1] = unmethylated, [2] = total

bismark2report

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## You should have received a copy of the GNU General Public License
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## along with this program. If not, see <http://www.gnu.org/licenses/>.
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my $bismark2report_version = 'v0.25.0';
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my $bismark2report_version = 'v0.25.1';
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my (@alignment_reports,@dedup_reports,@splitting_reports,@mbias_reports,@nuc_reports);
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my ($output_dir,$verbose,$manual_output_file) = process_commandline();

bismark2summary

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## You should have received a copy of the GNU General Public License
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## along with this program. If not, see <http://www.gnu.org/licenses/>.
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my $bismark_version = '0.25.0';
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my $bismark_version = '0.25.1';
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# Last modified 09 11 2020
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my ($report_basename,$page_title,$verbose) = process_commandline();

bismark_genome_preparation

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my %freqs;
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my $bismark_version = 'v0.25.0';
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my $bismark_version = 'v0.25.1';
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my $copyright_date = '2010-25';
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my $last_modified = "19 May 2022";
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bismark_methylation_extractor

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my %counting;
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my $parent_dir = getcwd();
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my %fhs;
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my %fhs;
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my $version = 'v0.25.0';
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my $version = 'v0.25.1';
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my ($ignore,$genomic_fasta,$single,$paired,$full,$report,$no_overlap,$merge_non_CpG,$output_dir,$no_header,$bedGraph,$remove,$coverage_threshold,$counts,$cytosine_report,$genome_folder,$zero,$CpG_only,$CX_context,$split_by_chromosome,$sort_size,$samtools_path,$gzip,$ignore_r2,$mbias_off,$mbias_only,$gazillion,$ample_mem,$ignore_3prime,$ignore_3prime_r2,$multicore,$yacht,$ucsc) = process_commandline();
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### only needed for bedGraph output

coverage2cytosine

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my %chromosomes; # storing sequence information of all chromosomes/scaffolds
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my %processed; # keeping a record of which chromosomes have been processed
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my %context_summary; # storing methylation values for all contexts for NOMe-seq or scNMT-experiments
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my $coverage2cytosine_version = 'v0.25.0';
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my $coverage2cytosine_version = 'v0.25.1';
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my ($output_dir,$genome_folder,$zero,$CpG_only,$CX_context,$split_by_chromosome,$parent_dir,$coverage_infile,$cytosine_out,$merge_CpGs,$gc_context,$gzip,$tetra,$nome,$disco,$threshold,$drach) = process_commandline();
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