chipseq/modules/local/star_genomegenerate/main.nf at 84f4b8bc5b9ee5caab5f8e8d11810a67159f9a04 · Kevin-Brockers/chipseq · GitHub

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process STAR_GENOMEGENERATE {
    tag "$fasta"
    label 'process_high'

    // Note: 2.7X indices incompatible with AWS iGenomes.
    conda "bioconda::star=2.6.1d bioconda::samtools=1.10 conda-forge::gawk=5.1.0"
    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
        'https://depot.galaxyproject.org/singularity/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:59cdd445419f14abac76b31dd0d71217994cbcc9-0' :
        'biocontainers/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:59cdd445419f14abac76b31dd0d71217994cbcc9-0' }"

    input:
    path fasta
    path gtf

    output:
    path "star"        , emit: index
    path "versions.yml", emit: versions

    when:
    task.ext.when == null || task.ext.when

    script:
    def args   = (task.ext.args ?: '').tokenize()
    def memory = task.memory ? "--limitGenomeGenerateRAM ${task.memory.toBytes() - 100000000}" : ''
    if (args.contains('--genomeSAindexNbases')) {
        """
        mkdir star
        STAR \\
            --runMode genomeGenerate \\
            --genomeDir star/ \\
            --genomeFastaFiles $fasta \\
            --sjdbGTFfile $gtf \\
            --runThreadN $task.cpus \\
            $memory \\
            ${args.join(' ')}
        cat <<-END_VERSIONS > versions.yml
        "${task.process}":
            star: \$(STAR --version | sed -e "s/STAR_//g")
        END_VERSIONS
        """
    } else {
        """
        samtools faidx $fasta
        NUM_BASES=`gawk '{sum = sum + \$2}END{if ((log(sum)/log(2))/2 - 1 > 14) {printf "%.0f", 14} else {printf "%.0f", (log(sum)/log(2))/2 - 1}}' ${fasta}.fai`
        mkdir star
        STAR \\
            --runMode genomeGenerate \\
            --genomeDir star/ \\
            --genomeFastaFiles $fasta \\
            --sjdbGTFfile $gtf \\
            --runThreadN $task.cpus \\
            --genomeSAindexNbases \$NUM_BASES \\
            $memory \\
            ${args.join(' ')}
        cat <<-END_VERSIONS > versions.yml
        "${task.process}":
            star: \$(STAR --version | sed -e "s/STAR_//g")
        END_VERSIONS
        """
    }
}