NVIDIA
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diff --git a/‎sub-packages/bionemo-evo2/src/bionemo/evo2/data/sharded_eden_dataloader.md‎
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-# SPDX-FileCopyrightText: Copyright (c) 2024 NVIDIA CORPORATION & AFFILIATES. All rights reserved.
-# SPDX-FileCopyrightText: Copyright (c) 2024 Arc Institute. All rights reserved.
-# SPDX-FileCopyrightText: Copyright (c) 2024 Michael Poli. All rights reserved.
-# SPDX-FileCopyrightText: Copyright (c) 2024 Stanford University. All rights reserved
+# SPDX-FileCopyrightText: Copyright (c) 2025 NVIDIA CORPORATION & AFFILIATES. All rights reserved.
 # SPDX-License-Identifier: LicenseRef-Apache2
 #
 # Licensed under the Apache License, Version 2.0 (the "License");
 
@@ -0,0 +1,282 @@
+# Sharded Eden DataLoader Implementation
+
+## Overview
+
+The `sharded_eden_dataloader.py` implements a dataloader for genomic sequences that uses pre-computed data structures and SQLite databases for efficient data access. This implementation is designed to significantly reduce the computational overhead during training by moving expensive operations to a pre-processing phase.
+
+## Key Features
+
+### 1. Split-Specific Window Databases
+
+- **Sharded**: Uses separate pre-computed window databases for each split:
+  - `train_window_db_path`: SQLite database containing window mappings for training data
+  - `val_window_db_path`: SQLite database containing window mappings for validation data
+  - `test_window_db_path`: SQLite database containing window mappings for test data
+
+### 2. SQLite Database Storage
+
+- **Sharded**: Uses SQLite databases organized by sample:
+  - **Per-Sample Sequence Databases**: Each sample has its own SQLite file at `sequence_db_dir/<sample_id>/glm_dataset_<sample_id>.sqlite`
+  - **Split-Specific Window Databases**: Pre-computed window mappings stored in separate databases for each data split
+
+### 3. Virtual Window Pre-computation
+
+- **Sharded**: Window mappings are pre-computed from Parquet files and stored in split-specific databases
+
+## Sequence ID Format
+
+Sequence IDs follow a specific format: `BCR__ECT-SAMPLE1__CT1-1`
+
+The sample ID can be extracted using: `extract_sample_id(sequence_id)` which implements `".".join(sequence_id.split("__")[1].split("-")[1:])` (returns `SAMPLE1`)
+
+## Database Schema
+
+### Per-Sample Sequence Database
+
+Each sample has its own SQLite file with the following schema:
+
+```sql
+CREATE TABLE sequences (
+    contig_id TEXT PRIMARY KEY,
+    nt_sequence TEXT NOT NULL
+);
+```
+
+### Split-Specific Window Database
+
+Each split (train/validation/test) has its own window database:
+
+```sql
+CREATE TABLE metadata (
+    key TEXT PRIMARY KEY,
+    value INTEGER NOT NULL
+);
+
+CREATE TABLE window_mappings (
+    window_idx INTEGER PRIMARY KEY,
+    sequence_id TEXT NOT NULL,
+    window_in_seq_idx INTEGER NOT NULL
+);
+CREATE INDEX idx_sequence_id ON window_mappings(sequence_id);
+```
+
+The metadata table stores the `window_size` and `stride` parameters used during pre-computation.
+
+## Directory Structure
+
+```
+sequence_db_dir/
+├── SAMPLE1/
+│   └── glm_dataset_SAMPLE1.sqlite
+├── SAMPLE2/
+│   └── glm_dataset_SAMPLE2.sqlite
+├── SAMPLE3/
+│   └── glm_dataset_SAMPLE3.sqlite
+└── ...
+
+Window databases (separate files):
+├── train_windows.db
+├── val_windows.db
+└── test_windows.db
+```
+
+## Usage Example
+
+```python
+from bionemo.evo2.run.sharded_eden_dataloader import ShardedEdenDataModule
+
+# Create the data module
+data_module = ShardedEdenDataModule(
+    sequence_db_dir="path/to/sequence_db_dir",  # Directory containing sample folders
+    train_window_db_path="path/to/train_windows.db",
+    val_window_db_path="path/to/val_windows.db",
+    test_window_db_path="path/to/test_windows.db",
+    seq_length=8192,
+    micro_batch_size=1,
+    global_batch_size=4,
+    num_workers=8,
+    rc_aug=True,
+    use_control_tags=True,
+)
+
+# Use with PyTorch Lightning trainer
+trainer = pl.Trainer(...)
+trainer.fit(model, data_module)
+```
+
+## Pre-processing Workflow
+
+### 1. Create Sample Sequence Databases
+
+For each sample, create its SQLite database:
+
+```python
+import sqlite3
+import os
+
+
+def create_sample_database(sample_id, sequences, output_dir):
+    """Create SQLite database for a single sample."""
+    # Create sample directory
+    sample_dir = os.path.join(output_dir, sample_id)
+    os.makedirs(sample_dir, exist_ok=True)
+
+    # Create database
+    db_path = os.path.join(sample_dir, f"glm_dataset_{sample_id}.sqlite")
+    conn = sqlite3.connect(db_path)
+    cursor = conn.cursor()
+
+    # Create table
+    cursor.execute(
+        """
+        CREATE TABLE sequences (
+            contig_id TEXT PRIMARY KEY,
+            nt_sequence TEXT NOT NULL
+        )
+    """
+    )
+
+    # Insert sequences for this sample
+    for seq_id, sequence in sequences:
+        cursor.execute(
+            "INSERT INTO sequences (contig_id, nt_sequence) VALUES (?, ?)",
+            (seq_id, sequence),
+        )
+
+    conn.commit()
+    conn.close()
+
+
+# Example usage
+# Group sequences by sample_id
+from collections import defaultdict
+
+sequences_by_sample = defaultdict(list)
+for seq_id, sequence in all_sequences:  # all_sequences is your data
+    sample_id = extract_sample_id(seq_id)
+    sequences_by_sample[sample_id].append((seq_id, sequence))
+
+# Create database for each sample
+for sample_id, sequences in sequences_by_sample.items():
+    create_sample_database(sample_id, sequences, "path/to/sequence_db_dir")
+```
+
+### 2. Create Split Data Files
+
+Create Parquet files for each split containing sequence metadata:
+
+```python
+import polars as pl
+
+# Create train split Parquet file
+train_data = pl.DataFrame(
+    {
+        "contig_id": ["BCR__ECT-SAMPLE1__CT1-1", "BCR__ECT-SAMPLE1__CT1-2", ...],
+        "length": [1500, 2000, ...],  # sequence lengths
+    }
+)
+train_data.write_parquet("train_split.parquet")
+
+# Similarly for validation and test splits
+val_data = pl.DataFrame(
+    {"contig_id": ["BCR__ECT-SAMPLE2__CT1-1", ...], "length": [1800, ...]}
+)
+val_data.write_parquet("val_split.parquet")
+
+test_data = pl.DataFrame(
+    {"contig_id": ["BCR__ECT-SAMPLE3__CT1-1", ...], "length": [1600, ...]}
+)
+test_data.write_parquet("test_split.parquet")
+```
+
+### 3. Create Window Mappings Databases using CLI
+
+The package includes a CLI tool for pre-computing the window databases:
+
+```bash
+# Pre-compute window mappings for training split
+python -m bionemo.evo2.run.sharded_eden_dataloader precompute \
+    train_split.parquet \
+    train_windows.db \
+    --window-size 8192 \
+    --stride 7992
+
+# Pre-compute window mappings for validation split
+python -m bionemo.evo2.run.sharded_eden_dataloader precompute \
+    val_split.parquet \
+    val_windows.db \
+    --window-size 8192 \
+    --stride 7992
+
+# Pre-compute window mappings for test split
+python -m bionemo.evo2.run.sharded_eden_dataloader precompute \
+    test_split.parquet \
+    test_windows.db \
+    --window-size 8192 \
+    --stride 7992
+```
+
+## Implementation Details
+
+### Key Components
+
+1. **ShardedEdenDataModule**:
+
+   - Uses separate window databases for each split (train/val/test)
+   - Manages per-sample SQLite file paths
+   - Creates datasets with directory and database paths
+   - Handles distributed training setup with Megatron integration
+
+2. **ShardedEdenDataset**:
+
+   - Automatically discovers sample SQLite files from directory structure
+   - Maps sequence IDs to appropriate sample databases using `extract_sample_id()`
+   - Pre-opens all database connections for performance
+   - Attaches window database to each sequence connection for efficient JOINs
+   - Implements sequence caching with connection pooling
+   - Maintains compatibility with original tokenization and formatting logic
+   - Optional window access logging for performance analysis
+
+3. **CLI Tool**:
+
+   - `precompute`: Creates window databases from Parquet files
+
+### Advanced Features
+
+#### Window Access Logging
+
+Enable detailed logging of window access patterns:
+
+```python
+dataset = ShardedEdenDataset(
+    # ... other parameters ...
+    log_windows=True,
+    log_dir="sequence_logs",
+)
+```
+
+This creates CSV logs tracking which windows are accessed, useful for analyzing data loading patterns.
+
+#### Connection Management
+
+- All database connections are pre-opened during initialization for performance
+- Database connections are pooled and reused per sample
+- Sequence data is fetched on-demand using SQL SUBSTR for memory efficiency
+- Position IDs are shared across instances to reduce memory usage
+- Connections are properly closed when dataset is destroyed
+
+#### Metadata Validation
+
+The implementation validates that window databases were created with compatible parameters:
+
+- Checks stored `window_size` matches dataset `seq_length`
+- Checks stored `stride` matches dataset `stride`
+- Provides clear error messages for mismatches
+
+### Error Handling
+
+- Validates sample SQLite files exist during initialization
+- Handles missing sequences gracefully with informative error messages
+- Ensures proper cleanup of database connections
+- Provides detailed debugging information for database issues
+- Validates Parquet file schema during pre-computation
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`1`		`-# SPDX-FileCopyrightText: Copyright (c) 2024 NVIDIA CORPORATION & AFFILIATES. All rights reserved.`
`2`		`-# SPDX-FileCopyrightText: Copyright (c) 2024 Arc Institute. All rights reserved.`
`3`		`-# SPDX-FileCopyrightText: Copyright (c) 2024 Michael Poli. All rights reserved.`
`4`		`-# SPDX-FileCopyrightText: Copyright (c) 2024 Stanford University. All rights reserved`
	`1`	`+# SPDX-FileCopyrightText: Copyright (c) 2025 NVIDIA CORPORATION & AFFILIATES. All rights reserved.`
`5`	`2`	`# SPDX-License-Identifier: LicenseRef-Apache2`
`6`	`3`	`#`
`7`	`4`	`# Licensed under the Apache License, Version 2.0 (the "License");`