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Merge branch 'main' of github.com:SPAAM-community/intro-to-ancient-metagenomics-book
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authentication.qmd

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@@ -475,7 +475,7 @@ For custom reference genomes not covered by NCBI, their accession IDs and the co
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The `ngsLCA` program considers a chosen similarity interval between each read and its reference in the generated bam/sam file.
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The similarity can be set as an edit distance `[-editdist[min/max]]`, i.e., number of mismatches between the read to reference genome, or as a similarity distance `[-simscore[low/high]]`, i.e., percentage of mismatches between the read to reference genome.
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The main files produced by this command have the extensions `.bdamage.gz`, `lca.gz` and 'stat.gz'.
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The main files produced by this command have the extensions `.bdamage.gz`, `lca.gz` and `stat.gz`.
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The first consists of a nucleotide misincorporation matrix (also called **mismatch matrix**) which represents the nucleotide substitution counts across the reads (@tbl-authentication-examplecodetable2).
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The lca file reports the sequences analysed and their taxonomic paths, while the stat file includes other statistics (gc content, fragment length).
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The best documentation is currently found in the –help function.
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:::
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metaDMG is installed in the conda environment 'authentication`. If not activated yet, we run
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metaDMG is installed in the conda environment `authentication`. If not activated yet, we run
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```bash
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conda activate authentication

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