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Benchmarking adapter and quality trimming tools

Original implementation by Brian Bushnell (2014): http://seqanswers.com/forums/showthread.php?t=42776

The following tools are compared:

Fake adapters, "gruseq"

The comparison adds fake truseq adapters, "gruseq", provided by Brian Bushnell, downloaded from: http://seqanswers.com/forums/attachment.php?attachmentid=2993&d=1398383571

Test data

A subset of a single sample is from SRA is provided in the repo. Comment/uncomment the download_FASTQ setting in the config/config.yaml to download the complete sample from SRA instead. Feel free to replace the URL with whatever FASTQ file you want.

Results

These plots are produced by the workflow, one for benchmarking results (Snakemake runs each tool three times to assess runtime, CPU and RAM usage), and one plot with adapter removal performance.

Benchmark results

Adapter removal performance

Running

Run the benchmarking workflow with something like:

snakemake --use-conda --cores 8