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bam_peaks_call_qc_annotate_macs3_homer
bed_consensus_quantify_qc_bedtools_featurecounts_deseq2 Expand file tree Collapse file tree Original file line number Diff line number Diff line change @@ -66,7 +66,6 @@ workflow NFCORE_CHIPSEQ {
6666 params. chromap_index,
6767 params. star_index,
6868 )
69- ch_versions = ch_versions. mix(PREPARE_GENOME . out. versions)
7069
7170 //
7271 // WORKFLOW: Run nf-core/chipseq workflow
Original file line number Diff line number Diff line change @@ -81,7 +81,6 @@ workflow BAM_PEAKS_CALL_QC_ANNOTATE_MACS3_HOMER {
8181 ch_peak_count_header_multiqc,
8282 ch_frip_score_multiqc
8383 )
84- ch_versions = ch_versions. mix(MULTIQC_CUSTOM_PEAKS . out. versions. first())
8584
8685 ch_homer_annotatepeaks = channel. empty()
8786 ch_plot_macs3_qc_txt = channel. empty()
@@ -99,7 +98,6 @@ workflow BAM_PEAKS_CALL_QC_ANNOTATE_MACS3_HOMER {
9998 ch_gtf
10099 )
101100 ch_homer_annotatepeaks = HOMER_ANNOTATEPEAKS . out. txt
102- ch_versions = ch_versions. mix(HOMER_ANNOTATEPEAKS . out. versions. first())
103101
104102 if (! skip_peak_qc) {
105103 //
@@ -146,6 +144,4 @@ workflow BAM_PEAKS_CALL_QC_ANNOTATE_MACS3_HOMER {
146144 plot_homer_annotatepeaks_txt = ch_plot_homer_annotatepeaks_txt // channel: [ txt ]
147145 plot_homer_annotatepeaks_pdf = ch_plot_homer_annotatepeaks_pdf // channel: [ pdf ]
148146 plot_homer_annotatepeaks_tsv = ch_plot_homer_annotatepeaks_tsv // channel: [ tsv ]
149-
150- versions = ch_versions // channel: [ versions.yml ]
151147}
Original file line number Diff line number Diff line change @@ -68,15 +68,13 @@ workflow BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2 {
6868 ch_fasta,
6969 ch_gtf
7070 )
71- ch_versions = ch_versions. mix(HOMER_ANNOTATEPEAKS . out. versions)
7271
7372 //
7473 // MODULE: Add boolean fields to annotated consensus peaks to aid filtering
7574 //
7675 ANNOTATE_BOOLEAN_PEAKS (
7776 MACS3_CONSENSUS . out. boolean_txt. join(HOMER_ANNOTATEPEAKS . out. txt, by : [0 ]),
7877 )
79- ch_versions = ch_versions. mix(ANNOTATE_BOOLEAN_PEAKS . out. versions)
8078 }
8179
8280 // Create channels: [ meta, [ ip_bams ], saf ]
@@ -128,7 +126,6 @@ workflow BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2 {
128126 ch_deseq2_qc_dists_multiqc = DESEQ2_QC . out. dists_multiqc
129127 ch_deseq2_qc_log = DESEQ2_QC . out. log
130128 ch_deseq2_qc_size_factors = DESEQ2_QC . out. size_factors
131- ch_versions = ch_versions. mix(DESEQ2_QC . out. versions)
132129 }
133130
134131 emit :
@@ -151,6 +148,4 @@ workflow BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2 {
151148 deseq2_qc_dists_multiqc = ch_deseq2_qc_dists_multiqc // channel: [ txt ]
152149 deseq2_qc_log = ch_deseq2_qc_log // channel: [ txt ]
153150 deseq2_qc_size_factors = ch_deseq2_qc_size_factors // channel: [ txt ]
154-
155- versions = ch_versions // channel: [ versions.yml ]
156151}
Original file line number Diff line number Diff line change @@ -18,7 +18,6 @@ workflow INPUT_CHECK {
1818
1919 emit :
2020 reads // channel: [ val(meta), [ reads ] ]
21- versions = SAMPLESHEET_CHECK . out. versions // channel: [ versions.yml ]
2221}
2322
2423// Function to get list of [ meta, [ fastq_1, fastq_2 ] ]
Original file line number Diff line number Diff line change @@ -102,7 +102,6 @@ workflow PREPARE_GENOME {
102102
103103 if (make_bed) {
104104 ch_gene_bed = GTF2BED (ch_gtf). bed
105- ch_versions = ch_versions. mix(GTF2BED . out. versions)
106105 } else {
107106 if (gene_bed. endsWith(' .gz' )) {
108107 ch_gene_bed = GUNZIP_GENE_BED ([[:], gene_bed]). gunzip. map { it[1 ] }
@@ -207,5 +206,4 @@ workflow PREPARE_GENOME {
207206 bowtie2_index = ch_bowtie2_index // path: bowtie2/index/
208207 chromap_index = ch_chromap_index // path: genome.index
209208 star_index = ch_star_index // path: star/index/
210- versions = ch_versions // path: versions.yml
211209}
Original file line number Diff line number Diff line change @@ -462,7 +462,6 @@ workflow CHIPSEQ {
462462 params. skip_peak_annotation,
463463 params. skip_peak_qc
464464 )
465- ch_versions = ch_versions. mix(BAM_PEAKS_CALL_QC_ANNOTATE_MACS3_HOMER . out. versions)
466465
467466 //
468467 // Consensus peaks analysis
@@ -502,7 +501,6 @@ workflow CHIPSEQ {
502501 ch_subreadfeaturecounts_multiqc = BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2 . out. featurecounts_summary
503502 ch_deseq2_pca_multiqc = BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2 . out. deseq2_qc_pca_multiqc
504503 ch_deseq2_clustering_multiqc = BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2 . out. deseq2_qc_dists_multiqc
505- ch_versions = ch_versions. mix(BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2 . out. versions)
506504 }
507505
508506 //
@@ -518,14 +516,11 @@ workflow CHIPSEQ {
518516 ch_macs3_consensus_bed_lib. collect{it[1 ]}. ifEmpty([]),
519517 ch_macs3_consensus_txt_lib. collect{it[1 ]}. ifEmpty([])
520518 )
521- ch_versions = ch_versions. mix(IGV . out. versions)
522519 }
523520
524521 //
525522 // Collate and save software versions
526523 //
527- ch_versions = ch_versions. mix(INPUT_CHECK . out. versions)
528- ch_versions = ch_versions. mix(BAM_PEAKS_CALL_QC_ANNOTATE_MACS3_HOMER . out. versions)
529524
530525 ch_collated_versions = softwareVersionsToYAML(ch_versions. mix(topic_versions. versions_file))
531526 .mix(topic_versions_string)
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