@@ -62,91 +62,91 @@ jobs:
6262 if [[ $NXF_VER = '' ]]; then sleep 1200; fi
6363 - name : BASIC Run the basic pipeline with directly supplied single-end FASTQ
6464 run : |
65- nextflow run ${GITHUB_WORKSPACE} -profile test ,docker --input 'data/testdata/Mammoth/fastq/*_R1_*.fq.gz' --single_end
65+ nextflow run ${GITHUB_WORKSPACE} -profile test_direct ,docker --input 'data/testdata/Mammoth/fastq/*_R1_*.fq.gz' --single_end
6666 - name : BASIC Run the basic pipeline with directly supplied paired-end FASTQ
6767 run : |
68- nextflow run ${GITHUB_WORKSPACE} -profile test ,docker --input 'data/testdata/Mammoth/fastq/*_{R1,R2}_*tengrand.fq.gz'
68+ nextflow run ${GITHUB_WORKSPACE} -profile test_direct ,docker --input 'data/testdata/Mammoth/fastq/*_{R1,R2}_*tengrand.fq.gz'
6969 - name : BASIC Run the basic pipeline with supplied --input BAM
7070 run : |
71- nextflow run ${GITHUB_WORKSPACE} -profile test ,docker --input 'data/testdata/Mammoth/bam/*_R1_*.bam' --bam --single_end
71+ nextflow run ${GITHUB_WORKSPACE} -profile test_direct ,docker --input 'data/testdata/Mammoth/bam/*_R1_*.bam' --bam --single_end
7272 - name : BASIC Run the basic pipeline with the test profile with, PE/SE, bwa aln
7373 run : |
74- nextflow run ${GITHUB_WORKSPACE} -profile test_tsv ,docker --save_reference
74+ nextflow run ${GITHUB_WORKSPACE} -profile test ,docker --save_reference
7575 - name : REFERENCE Basic workflow, with supplied indices
7676 run : |
77- nextflow run ${GITHUB_WORKSPACE} -profile test_tsv ,docker --bwa_index 'results/reference_genome/bwa_index/BWAIndex/' --fasta_index 'https://github.com/nf-core/test-datasets/blob/eager/reference/Mammoth/Mammoth_MT_Krause.fasta.fai'
77+ nextflow run ${GITHUB_WORKSPACE} -profile test ,docker --bwa_index 'results/reference_genome/bwa_index/BWAIndex/' --fasta_index 'https://github.com/nf-core/test-datasets/blob/eager/reference/Mammoth/Mammoth_MT_Krause.fasta.fai'
7878 - name : REFERENCE Run the basic pipeline with FastA reference with `fna` extension
7979 run : |
8080 nextflow run ${GITHUB_WORKSPACE} -profile test_tsv_fna,docker
8181 - name : REFERENCE Test with zipped reference input
8282 run : |
83- nextflow run ${GITHUB_WORKSPACE} -profile test_tsv ,docker --fasta 'https://github.com/nf-core/test-datasets/raw/eager/reference/Mammoth/Mammoth_MT_Krause.fasta.gz'
83+ nextflow run ${GITHUB_WORKSPACE} -profile test ,docker --fasta 'https://github.com/nf-core/test-datasets/raw/eager/reference/Mammoth/Mammoth_MT_Krause.fasta.gz'
8484 - name : FASTP Test fastp complexity filtering
8585 run : |
86- nextflow run ${GITHUB_WORKSPACE} -profile test_tsv ,docker --complexity_filter_poly_g
86+ nextflow run ${GITHUB_WORKSPACE} -profile test ,docker --complexity_filter_poly_g
8787 - name : ADAPTERREMOVAL Test skip paired end collapsing
8888 run : |
89- nextflow run ${GITHUB_WORKSPACE} -profile test_tsv ,docker --skip_collapse
89+ nextflow run ${GITHUB_WORKSPACE} -profile test ,docker --skip_collapse
9090 - name : ADAPTERREMOVAL Test paired end collapsing but no trimming
9191 run : |
9292 nextflow run ${GITHUB_WORKSPACE} -profile test_tsv_pretrim,docker --skip_trim
9393 - name : ADAPTERREMOVAL Run the basic pipeline with paired end data without adapterRemoval
9494 run : |
95- nextflow run ${GITHUB_WORKSPACE} -profile test_tsv ,docker --skip_adapterremoval
95+ nextflow run ${GITHUB_WORKSPACE} -profile test ,docker --skip_adapterremoval
9696 - name : ADAPTERREMOVAL Run the basic pipeline with preserve5p end option
9797 run : |
98- nextflow run ${GITHUB_WORKSPACE} -profile test_tsv ,docker --preserve5p
98+ nextflow run ${GITHUB_WORKSPACE} -profile test ,docker --preserve5p
9999 - name : ADAPTERREMOVAL Run the basic pipeline with merged only option
100100 run : |
101- nextflow run ${GITHUB_WORKSPACE} -profile test_tsv ,docker --mergedonly
101+ nextflow run ${GITHUB_WORKSPACE} -profile test ,docker --mergedonly
102102 - name : ADAPTERREMOVAL Run the basic pipeline with preserve5p end and merged reads only options
103103 run : |
104- nextflow run ${GITHUB_WORKSPACE} -profile test_tsv ,docker --preserve5p --mergedonly
104+ nextflow run ${GITHUB_WORKSPACE} -profile test ,docker --preserve5p --mergedonly
105105 - name : ADAPTER LIST Run the basic pipeline using an adapter list
106106 run : |
107- nextflow run ${GITHUB_WORKSPACE} -profile test_tsv ,docker --clip_adapters_list 'https://github.com/nf-core/test-datasets/raw/eager/databases/adapters/adapter-list.txt'
107+ nextflow run ${GITHUB_WORKSPACE} -profile test ,docker --clip_adapters_list 'https://github.com/nf-core/test-datasets/raw/eager/databases/adapters/adapter-list.txt'
108108 - name : ADAPTER LIST Run the basic pipeline using an adapter list, skipping adapter removal
109109 run : |
110- nextflow run ${GITHUB_WORKSPACE} -profile test_tsv ,docker --clip_adapters_list 'https://github.com/nf-core/test-datasets/raw/eager/databases/adapters/adapter-list.txt' --skip_adapterremoval
110+ nextflow run ${GITHUB_WORKSPACE} -profile test ,docker --clip_adapters_list 'https://github.com/nf-core/test-datasets/raw/eager/databases/adapters/adapter-list.txt' --skip_adapterremoval
111111 - name : POST_AR_FASTQ_TRIMMING Run the basic pipeline post-adapterremoval FASTQ trimming
112112 run : |
113- nextflow run ${GITHUB_WORKSPACE} -profile test_tsv ,docker --run_post_ar_trimming
113+ nextflow run ${GITHUB_WORKSPACE} -profile test ,docker --run_post_ar_trimming
114114 - name : POST_AR_FASTQ_TRIMMING Run the basic pipeline post-adapterremoval FASTQ trimming, but skip adapterremoval
115115 run : |
116- nextflow run ${GITHUB_WORKSPACE} -profile test_tsv ,docker --run_post_ar_trimming --skip_adapterremoval
116+ nextflow run ${GITHUB_WORKSPACE} -profile test ,docker --run_post_ar_trimming --skip_adapterremoval
117117 - name : MAPPER_CIRCULARMAPPER Test running with CircularMapper
118118 run : |
119- nextflow run ${GITHUB_WORKSPACE} -profile test_tsv ,docker --mapper 'circularmapper' --circulartarget 'NC_007596.2'
119+ nextflow run ${GITHUB_WORKSPACE} -profile test ,docker --mapper 'circularmapper' --circulartarget 'NC_007596.2'
120120 - name : MAPPER_BWAMEM Test running with BWA Mem
121121 run : |
122- nextflow run ${GITHUB_WORKSPACE} -profile test_tsv ,docker --mapper 'bwamem'
122+ nextflow run ${GITHUB_WORKSPACE} -profile test ,docker --mapper 'bwamem'
123123 - name : MAPPER_BT2 Test running with BowTie2
124124 run : |
125- nextflow run ${GITHUB_WORKSPACE} -profile test_tsv ,docker --mapper 'bowtie2' --bt2_alignmode 'local' --bt2_sensitivity 'sensitive' --bt2n 1 --bt2l 16 --bt2_trim5 1 --bt2_trim3 1
125+ nextflow run ${GITHUB_WORKSPACE} -profile test ,docker --mapper 'bowtie2' --bt2_alignmode 'local' --bt2_sensitivity 'sensitive' --bt2n 1 --bt2l 16 --bt2_trim5 1 --bt2_trim3 1
126126 - name : HOST_REMOVAL_FASTQ Run the basic pipeline with output unmapped reads as fastq
127127 run : |
128128 nextflow run ${GITHUB_WORKSPACE} -profile test_tsv_complex,docker --hostremoval_input_fastq
129129 - name : BAM_FILTERING Run basic mapping pipeline with mapping quality filtering, and unmapped export
130130 run : |
131- nextflow run ${GITHUB_WORKSPACE} -profile test_tsv ,docker --run_bam_filtering --bam_mapping_quality_threshold 37 --bam_unmapped_type 'fastq'
131+ nextflow run ${GITHUB_WORKSPACE} -profile test ,docker --run_bam_filtering --bam_mapping_quality_threshold 37 --bam_unmapped_type 'fastq'
132132 - name : BAM_FILTERING Run basic mapping pipeline with post-mapping length filtering
133133 run : |
134- nextflow run ${GITHUB_WORKSPACE} -profile test_tsv ,docker --clip_readlength 0 --run_bam_filtering --bam_filter_minreadlength 50
134+ nextflow run ${GITHUB_WORKSPACE} -profile test ,docker --clip_readlength 0 --run_bam_filtering --bam_filter_minreadlength 50
135135 - name : PRESEQ Run basic mapping pipeline with different preseq mode
136136 run : |
137- nextflow run ${GITHUB_WORKSPACE} -profile test_tsv ,docker --preseq_mode 'lc_extrap' --preseq_maxextrap 10000 --preseq_bootstrap 10
137+ nextflow run ${GITHUB_WORKSPACE} -profile test ,docker --preseq_mode 'lc_extrap' --preseq_maxextrap 10000 --preseq_bootstrap 10
138138 - name : DEDUPLICATION Test with dedup
139139 run : |
140- nextflow run ${GITHUB_WORKSPACE} -profile test_tsv ,docker --dedupper 'dedup' --dedup_all_merged
140+ nextflow run ${GITHUB_WORKSPACE} -profile test ,docker --dedupper 'dedup' --dedup_all_merged
141141 - name : BEDTOOLS Test bedtools feature annotation
142142 run : |
143- nextflow run ${GITHUB_WORKSPACE} -profile test_tsv ,docker --run_bedtools_coverage --anno_file 'https://github.com/nf-core/test-datasets/raw/eager/reference/Mammoth/Mammoth_MT_Krause.gff3'
143+ nextflow run ${GITHUB_WORKSPACE} -profile test ,docker --run_bedtools_coverage --anno_file 'https://github.com/nf-core/test-datasets/raw/eager/reference/Mammoth/Mammoth_MT_Krause.gff3'
144144 - name : GENOTYPING_HC Test running GATK HaplotypeCaller
145145 run : |
146146 nextflow run ${GITHUB_WORKSPACE} -profile test_tsv_fna,docker --run_genotyping --genotyping_tool 'hc' --gatk_hc_out_mode 'EMIT_ALL_ACTIVE_SITES' --gatk_hc_emitrefconf 'BP_RESOLUTION'
147147 - name : GENOTYPING_FB Test running FreeBayes
148148 run : |
149- nextflow run ${GITHUB_WORKSPACE} -profile test_tsv ,docker --run_genotyping --genotyping_tool 'freebayes'
149+ nextflow run ${GITHUB_WORKSPACE} -profile test ,docker --run_genotyping --genotyping_tool 'freebayes'
150150 - name : GENOTYPING_PC Test running pileupCaller
151151 run : |
152152 nextflow run ${GITHUB_WORKSPACE} -profile test_tsv_humanbam,docker --run_genotyping --genotyping_tool 'pileupcaller'
@@ -155,25 +155,25 @@ jobs:
155155 nextflow run ${GITHUB_WORKSPACE} -profile test_tsv_humanbam,docker --run_genotyping --genotyping_tool 'angsd'
156156 - name : GENOTYPING_BCFTOOLS Test running FreeBayes with bcftools stats turned on
157157 run : |
158- nextflow run ${GITHUB_WORKSPACE} -profile test_tsv ,docker --run_genotyping --genotyping_tool 'freebayes' --run_bcftools_stats
158+ nextflow run ${GITHUB_WORKSPACE} -profile test ,docker --run_genotyping --genotyping_tool 'freebayes' --run_bcftools_stats
159159 - name : SKIPPING Test checking all skip steps work i.e. input bam, skipping straight to genotyping
160160 run : |
161161 nextflow run ${GITHUB_WORKSPACE} -profile test_tsv_bam,docker --skip_fastqc --skip_adapterremoval --skip_deduplication --skip_qualimap --skip_preseq --skip_damage_calculation --run_genotyping --genotyping_tool 'freebayes'
162162 - name : TRIMBAM Test bamutils works alone
163163 run : |
164- nextflow run ${GITHUB_WORKSPACE} -profile test_tsv ,docker --run_trim_bam
164+ nextflow run ${GITHUB_WORKSPACE} -profile test ,docker --run_trim_bam
165165 - name : PMDTOOLS Test PMDtools works alone
166166 run : |
167- nextflow run ${GITHUB_WORKSPACE} -profile test_tsv ,docker --run_pmdtools
167+ nextflow run ${GITHUB_WORKSPACE} -profile test ,docker --run_pmdtools
168168 - name : GENOTYPING_UG AND MULTIVCFANALYZER Test running GATK UnifiedGenotyper and MultiVCFAnalyzer, additional VCFS
169169 run : |
170- nextflow run ${GITHUB_WORKSPACE} -profile test_tsv ,docker --run_genotyping --genotyping_tool 'ug' --gatk_ug_out_mode 'EMIT_ALL_SITES' --gatk_ug_genotype_model 'SNP' --run_multivcfanalyzer --additional_vcf_files 'https://raw.githubusercontent.com/nf-core/test-datasets/eager/testdata/Mammoth/vcf/JK2772_CATCAGTGAGTAGA_L008_R1_001.fastq.gz.tengrand.fq.combined.fq.mapped_rmdup.bam.unifiedgenotyper.vcf.gz' --write_allele_frequencies
170+ nextflow run ${GITHUB_WORKSPACE} -profile test ,docker --run_genotyping --genotyping_tool 'ug' --gatk_ug_out_mode 'EMIT_ALL_SITES' --gatk_ug_genotype_model 'SNP' --run_multivcfanalyzer --additional_vcf_files 'https://raw.githubusercontent.com/nf-core/test-datasets/eager/testdata/Mammoth/vcf/JK2772_CATCAGTGAGTAGA_L008_R1_001.fastq.gz.tengrand.fq.combined.fq.mapped_rmdup.bam.unifiedgenotyper.vcf.gz' --write_allele_frequencies
171171 - name : COMPLEX LANE/LIBRARY MERGING Test running lane and library merging prior to GATK UnifiedGenotyper and running MultiVCFAnalyzer
172172 run : |
173173 nextflow run ${GITHUB_WORKSPACE} -profile test_tsv_complex,docker --run_genotyping --genotyping_tool 'ug' --gatk_ug_out_mode 'EMIT_ALL_SITES' --gatk_ug_genotype_model 'SNP' --run_multivcfanalyzer
174174 - name : GENOTYPING_UG ON TRIMMED BAM Test
175175 run : |
176- nextflow run ${GITHUB_WORKSPACE} -profile test_tsv ,docker --run_genotyping --run_trim_bam --genotyping_source 'trimmed' --genotyping_tool 'ug' --gatk_ug_out_mode 'EMIT_ALL_SITES' --gatk_ug_genotype_model 'SNP'
176+ nextflow run ${GITHUB_WORKSPACE} -profile test ,docker --run_genotyping --run_trim_bam --genotyping_source 'trimmed' --genotyping_tool 'ug' --gatk_ug_out_mode 'EMIT_ALL_SITES' --gatk_ug_genotype_model 'SNP'
177177 - name : BAM_INPUT Run the basic pipeline with the bam input profile, skip AdapterRemoval as no convertBam
178178 run : |
179179 nextflow run ${GITHUB_WORKSPACE} -profile test_tsv_bam,docker --skip_adapterremoval
@@ -187,17 +187,17 @@ jobs:
187187 for i in index0.idx ref.db ref.idx ref.inf table0.db table0.idx taxonomy.idx taxonomy.map taxonomy.tre; do wget https://github.com/nf-core/test-datasets/raw/eager/databases/malt/"$i" -P databases/malt/; done
188188 - name : METAGENOMIC Run the basic pipeline but with unmapped reads going into MALT
189189 run : |
190- nextflow run ${GITHUB_WORKSPACE} -profile test_tsv ,docker --run_bam_filtering --bam_unmapped_type 'fastq' --run_metagenomic_screening --metagenomic_tool 'malt' --database "/home/runner/work/eager/eager/databases/malt/" --malt_sam_output
190+ nextflow run ${GITHUB_WORKSPACE} -profile test ,docker --run_bam_filtering --bam_unmapped_type 'fastq' --run_metagenomic_screening --metagenomic_tool 'malt' --database "/home/runner/work/eager/eager/databases/malt/" --malt_sam_output
191191 - name : METAGENOMIC Run the basic pipeline but low-complexity filtered reads going into MALT
192192 run : |
193- nextflow run ${GITHUB_WORKSPACE} -profile test_tsv ,docker --run_bam_filtering --bam_unmapped_type 'fastq' --run_metagenomic_screening --metagenomic_tool 'malt' --database "/home/runner/work/eager/eager/databases/malt/" --metagenomic_complexity_filter
193+ nextflow run ${GITHUB_WORKSPACE} -profile test ,docker --run_bam_filtering --bam_unmapped_type 'fastq' --run_metagenomic_screening --metagenomic_tool 'malt' --database "/home/runner/work/eager/eager/databases/malt/" --metagenomic_complexity_filter
194194 - name : MALTEXTRACT Download resource files
195195 run : |
196196 mkdir -p databases/maltextract
197197 for i in ncbi.tre ncbi.map; do wget https://github.com/rhuebler/HOPS/raw/0.33/Resources/"$i" -P databases/maltextract/; done
198198 - name : MALTEXTRACT Basic with MALT plus MaltExtract
199199 run : |
200- nextflow run ${GITHUB_WORKSPACE} -profile test_tsv ,docker --run_bam_filtering --bam_unmapped_type 'fastq' --run_metagenomic_screening --metagenomic_tool 'malt' --database "/home/runner/work/eager/eager/databases/malt" --run_maltextract --maltextract_ncbifiles "/home/runner/work/eager/eager/databases/maltextract/" --maltextract_taxon_list 'https://raw.githubusercontent.com/nf-core/test-datasets/eager/testdata/Mammoth/maltextract/MaltExtract_list.txt'
200+ nextflow run ${GITHUB_WORKSPACE} -profile test ,docker --run_bam_filtering --bam_unmapped_type 'fastq' --run_metagenomic_screening --metagenomic_tool 'malt' --database "/home/runner/work/eager/eager/databases/malt" --run_maltextract --maltextract_ncbifiles "/home/runner/work/eager/eager/databases/maltextract/" --maltextract_taxon_list 'https://raw.githubusercontent.com/nf-core/test-datasets/eager/testdata/Mammoth/maltextract/MaltExtract_list.txt'
201201 - name : METAGENOMIC Run the basic pipeline but with unmapped reads going into Kraken
202202 run : |
203203 nextflow run ${GITHUB_WORKSPACE} -profile test_tsv_kraken,docker --run_bam_filtering --bam_unmapped_type 'fastq'
@@ -212,4 +212,4 @@ jobs:
212212 nextflow run ${GITHUB_WORKSPACE} -profile test_tsv_humanbam,docker --skip_fastqc --skip_adapterremoval --skip_deduplication --skip_qualimap --skip_preseq --skip_damage_calculation --run_mtnucratio
213213 - name : RESCALING Run basic pipeline with basic pipeline but with mapDamage rescaling of BAM files. Note this will be slow
214214 run : |
215- nextflow run ${GITHUB_WORKSPACE} -profile test_tsv ,docker --run_mapdamage_rescaling --run_genotyping --genotyping_tool hc --genotyping_source 'rescaled'
215+ nextflow run ${GITHUB_WORKSPACE} -profile test ,docker --run_mapdamage_rescaling --run_genotyping --genotyping_tool hc --genotyping_source 'rescaled'
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