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Fix bwa mem indexing and re-update MQC SeqTools versions after git mess this morning
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CHANGELOG.md

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@@ -19,6 +19,7 @@ and this project adheres to [Semantic Versioning](http://semver.org/spec/v2.0.0.
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- Renamed a range of MultiQC general stats table headers to improve clarity, documentation has been updated accordingly
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- [#857](https://github.com/nf-core/eager/issues/857) Corrected samtools fastq flag to _retain_ read-pair information when converting off-target BAM files to fastq in paired-end mapping (❤ to @alexhbnr for reporting)
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- [#858](https://github.com/nf-core/eager/pull/858) Corrected tutorials to reflect updated BAM trimming flags (❤ to @marcel-keller for reporting)
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- [#858](https://github.com/nf-core/eager/pull/858) Corrected tutorials to reflect updated BAM trimming flags (❤ to @marcel-keller for reporting)
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### `Dependencies`
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environment.yml

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@@ -26,12 +26,12 @@ dependencies:
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- bioconda::qualimap=2.2.2d
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- bioconda::vcf2genome=0.91
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- bioconda::damageprofiler=0.4.9 # Don't upgrade - later versions don't allow java 8
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- bioconda::multiqc=1.11
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- bioconda::multiqc=1.12
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- bioconda::pmdtools=0.60
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- bioconda::bedtools=2.30.0
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- conda-forge::libiconv=1.16
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- conda-forge::pigz=2.6
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- bioconda::sequencetools=1.4.0.6
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- bioconda::sequencetools=1.5.2
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- bioconda::preseq=3.1.2
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- bioconda::fastp=0.20.1
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- bioconda::bamutil=1.0.15

main.nf

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@@ -1318,7 +1318,7 @@ process bwamem {
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if (!params.single_end && params.skip_collapse){
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"""
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bwa mem -t ${split_cpus} $fasta $r1 $r2 -R "@RG\\tID:ILLUMINA-${libraryid}\\tSM:${samplename}\\tPL:illumina\\tPU:ILLUMINA-${libraryid}-${seqtype}" | samtools sort -@ ${split_cpus} -O bam - > "${libraryid}"_"${seqtype}".mapped.bam
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samtools index ${size} -@ ${task.cpus} "${libraryid}".mapped.bam
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samtools index ${size} -@ ${task.cpus} "${libraryid}"_"${seqtype}".mapped.bam
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"""
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} else {
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"""

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