FastQCFastQC Report
Fri 2 Nov 2018
EXB015.A1701_S0_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEXB015.A1701_S0_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122977
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTATGCCGT2108217.143043008042156TruSeq Adapter, Index 11 (96% over 32bp)
CTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAA83886.820787626954634TruSeq Adapter, Index 11 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTA49674.0389666360376335TruSeq Adapter, Index 11 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTAT34062.7696235881506297TruSeq Adapter, Index 11 (97% over 37bp)
GTGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAG33612.733031379851517TruSeq Adapter, Index 11 (97% over 37bp)
CCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAG22111.7978971677630777TruSeq Adapter, Index 11 (97% over 37bp)
ACTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTA17491.4222171625588524TruSeq Adapter, Index 11 (97% over 37bp)
GAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGAT16671.3555380274360247TruSeq Adapter, Index 11 (97% over 37bp)
AGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATC14491.1782691072314335TruSeq Adapter, Index 11 (97% over 37bp)
GACTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTT14291.1620059035429389TruSeq Adapter, Index 11 (97% over 37bp)
CTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCT11840.9627816583588802TruSeq Adapter, Index 11 (97% over 37bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGT11200.9107394065556974TruSeq Adapter, Index 11 (97% over 37bp)
GAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC10400.8456865918017191TruSeq Adapter, Index 6 (100% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTATGCC8960.728591525244558TruSeq Adapter, Index 11 (97% over 34bp)
GGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGA8380.6814282345479236TruSeq Adapter, Index 11 (97% over 37bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTATGCCG6380.5187961976629777TruSeq Adapter, Index 11 (96% over 33bp)
ACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATC5560.45211706254014977TruSeq Adapter, Index 11 (97% over 37bp)
CTCTTCCGATCTGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGAT5360.4358538588516552TruSeq Adapter, Index 6 (97% over 36bp)
AGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTATGCCGTCT5120.41633801442546164TruSeq Adapter, Index 11 (96% over 30bp)
CTCTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGAT5110.41552485424103697TruSeq Adapter, Index 11 (97% over 37bp)
GTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCT4810.3911300487082951TruSeq Adapter, Index 11 (97% over 37bp)
GTGACTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC4800.3903168885238703TruSeq Adapter, Index 6 (100% over 36bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTATGC4280.3480325589337844TruSeq Adapter, Index 11 (97% over 35bp)
TGTGCTCTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTA3920.3187587922944941TruSeq Adapter, Index 11 (97% over 37bp)
GTGACTGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCT3840.31225351081909625TruSeq Adapter, Index 11 (97% over 37bp)
GAAGAGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAA3590.291924506208478TruSeq Adapter, Index 11 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTATG3560.28948502565520384TruSeq Adapter, Index 11 (97% over 36bp)
TGCTCTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAG3550.2886718654707791TruSeq Adapter, Index 11 (97% over 37bp)
GACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGAT3470.28216658399538125TruSeq Adapter, Index 11 (97% over 37bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGT3440.27972710344210705TruSeq Adapter, Index 11 (97% over 37bp)
AAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTATGCCGTC3330.27078234141343505TruSeq Adapter, Index 11 (96% over 31bp)
AAATTAATACGACTCACTATAGATCGGAAGAGCACACGTCTGAACTCCAG3000.24394805532741895Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAG2930.23825593403644582TruSeq Adapter, Index 11 (97% over 37bp)
TGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGA2930.23825593403644582TruSeq Adapter, Index 11 (97% over 37bp)
GAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTATGCCGTCTT2870.23337697292989748TruSeq Adapter, Index 11 (96% over 29bp)
AATTAATACGACTCACTATAGGGGTAGATCGGAAGAGCACACGTCTGAAC2860.23256381274547275Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGCTCTTCCGATCTGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAG2850.23175065256104802TruSeq Adapter, Index 6 (97% over 36bp)
TGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAG2660.21630060905697812TruSeq Adapter, Index 11 (97% over 37bp)
ACCCCTATAGTGAGTCGTATTAATTAGATCGGAAGAGCACACGTCTGAAC2180.17726892020459112Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCAGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTA2160.17564259983574165TruSeq Adapter, Index 6 (97% over 36bp)
GAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGAT2020.16425835725379542TruSeq Adapter, Index 11 (97% over 37bp)
CTCTTCCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC2000.16263203688494596TruSeq Adapter, Index 6 (100% over 36bp)
TGTGACTGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC1980.1610057165160965TruSeq Adapter, Index 6 (100% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1840.1496214739341503No Hit
TGTGCTCTTCCGATCTGAAGAGCACACGTCTGAACTCCAGTCACGCCTTA1760.14311619245875243TruSeq Adapter, Index 6 (97% over 36bp)
GTACAAAGAATTCCTGCAGAGATCGGAAGAGCACACGTCTGAACTCCAGT1750.14230303227432772Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGTGTGCTCTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCT1710.1390503915366288TruSeq Adapter, Index 11 (97% over 37bp)
AGATGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTAT1700.1382372313522041TruSeq Adapter, Index 11 (96% over 33bp)
TGACTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCT1700.1382372313522041TruSeq Adapter, Index 11 (97% over 37bp)
AGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTATGCCGTCTTC1580.12847930913910732TruSeq Adapter, Index 11 (96% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTAGATCGGAAGAGCACACGTCTGAA1520.12360034803255893Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTC1500.12197402766370948TruSeq Adapter, Index 11 (97% over 37bp)
TGTGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAA1480.12034770729486002TruSeq Adapter, Index 11 (97% over 37bp)
TGCTCTTCCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1430.11628190637273637Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCTTCCTGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC1310.10652398415963961TruSeq Adapter, Index 5 (97% over 35bp)
GATCGGAAGAGCGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1280.10408450360636541Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATCTAGTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTA1250.10164502305309121TruSeq Adapter, Index 11 (97% over 37bp)
CTCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAA1240.10083186286866651TruSeq Adapter, Index 11 (97% over 37bp)
CTGTGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTA1240.10083186286866651TruSeq Adapter, Index 11 (97% over 37bp)
GAAGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAG1230.10001870268424176TruSeq Adapter, Index 11 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)