FastQCFastQC Report
Fri 2 Nov 2018
EXB015.A1701_S0_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEXB015.A1701_S0_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122977
Sequences flagged as poor quality0
Sequence length76
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3034524.675345796368426No Hit
GAACTCCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTC74706.074306577652732Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTGTAGATC49434.0194507916114395Illumina Single End PCR Primer 1 (96% over 33bp)
CCAGTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCG30492.479325402311001Illumina Single End PCR Primer 1 (96% over 33bp)
GTGACTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCG20441.6620994169641479Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTG15591.2677167275181538Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCCAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACT15191.2351903201411647Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTGT14631.1896533498133797Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCCAGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGAC13241.0766240841783423Illumina Single End PCR Primer 1 (96% over 33bp)
CCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTGTA11840.9627816583588802Illumina Single End PCR Primer 1 (96% over 33bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTGTAGAT11650.9473316148548102Illumina Single End PCR Primer 1 (96% over 33bp)
GTGACTGGAGTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAG9840.8001496214739341Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGT7820.6358912642201388Illumina Single End PCR Primer 1 (96% over 33bp)
CCAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTGT5520.4488644218024509Illumina Single End PCR Primer 1 (96% over 33bp)
GAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTG5000.40658009221236485Illumina Single End PCR Primer 1 (96% over 33bp)
GAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTGTA4930.40088797092139183Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCCAGTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAG4140.3366483163518382Illumina Single End PCR Primer 1 (96% over 33bp)
GAAGAGCACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACT3530.28704554510192964Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCAGTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGA3470.28216658399538125Illumina Single End PCR Primer 1 (96% over 33bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTGTAGAT3400.2764744627044082Illumina Single End PCR Primer 1 (96% over 33bp)
ACGCTCTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTC3380.27484814233555865Illumina Single End PCR Primer 1 (96% over 33bp)
CCAGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTG3340.2715955015978598Illumina Single End PCR Primer 1 (96% over 33bp)
GAAGAGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCG3300.26834286086016085Illumina Single End PCR Primer 1 (96% over 33bp)
CCAGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGT2910.23662961366759638Illumina Single End PCR Primer 1 (96% over 33bp)
ATAGTGAGTCGTATTAATTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAG2840.23093749237662325Illumina Single End PCR Primer 1 (100% over 30bp)
ACCCCTATAGTGAGTCGTATTAATTAGATCGGAAGAGCGTCGTGTAGGGA2610.21223480813485446Illumina Single End PCR Primer 1 (100% over 25bp)
GAACTCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCG2580.2097953275815803Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCG2540.20654268684388138Illumina Single End PCR Primer 1 (96% over 33bp)
AATTAATACGACTCACTATAGGGGTAGATCGGAAGAGCGTCGTGTAGGGA2040.1658846776226449Illumina Single End PCR Primer 1 (100% over 25bp)
GAACTCAGTCACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAG1870.15206095448742446Illumina Single End PCR Primer 1 (96% over 33bp)
CTCTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTG1810.14718199338087612Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCGGAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAG1780.14474251282760192Illumina Single End PCR Primer 1 (96% over 33bp)
GAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTGTAG1730.14067671190547826Illumina Single End PCR Primer 1 (96% over 33bp)
CTGCAGGAATTCTTTGTACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT1640.1333582702456557Illumina Single End PCR Primer 1 (100% over 31bp)
GAACTCCAGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGA1570.1276661489546826Illumina Single End PCR Primer 1 (96% over 33bp)
GAAGAGCACACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGA1550.12603982858583312Illumina Single End PCR Primer 1 (96% over 33bp)
AGTTCAGACGTGTGCTCTTCCGATCTAGATCGGAAGAGCGTCGTGTAGGG1370.11140294526618799Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCAGTCACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTC1350.10977662489733853Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCGGAAGAGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCC1310.10652398415963961Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)