FastQCFastQC Report
Fri 2 Nov 2018
EXB015.A1701_S0_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEXB015.A1701_S0_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127540
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTATGCCGT2401018.825466520307355TruSeq Adapter, Index 11 (96% over 32bp)
CTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAA88176.913125294025403TruSeq Adapter, Index 11 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTA48913.834875333228791TruSeq Adapter, Index 11 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTAT35392.7748157440802887TruSeq Adapter, Index 11 (97% over 37bp)
GTGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAG34772.7262035439862005TruSeq Adapter, Index 11 (97% over 37bp)
CCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAG22111.733573780774659TruSeq Adapter, Index 11 (97% over 37bp)
ACTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTA18611.459150070566097TruSeq Adapter, Index 11 (97% over 37bp)
AGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATC15551.2192253410694682TruSeq Adapter, Index 11 (97% over 37bp)
GAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGAT14181.1118080602164027TruSeq Adapter, Index 11 (97% over 37bp)
GACTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTT11900.9330406147091108TruSeq Adapter, Index 11 (97% over 37bp)
GAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC10930.8569860435941665TruSeq Adapter, Index 6 (100% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTATGCC9990.7832836757095812TruSeq Adapter, Index 11 (97% over 34bp)
CTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCT9450.7409440175631175TruSeq Adapter, Index 11 (97% over 37bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGT8150.639015210914223TruSeq Adapter, Index 11 (97% over 37bp)
GGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGA8050.6311745334796927TruSeq Adapter, Index 11 (97% over 37bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTATGCCG7370.5778579269248864TruSeq Adapter, Index 11 (96% over 33bp)
ACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATC6330.4963148816057707TruSeq Adapter, Index 11 (97% over 37bp)
CTCTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGAT6050.4743609847890857TruSeq Adapter, Index 11 (97% over 37bp)
CTCTTCCGATCTGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGAT5910.4633840363807433TruSeq Adapter, Index 6 (97% over 36bp)
GTGACTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC5220.4092833620824839TruSeq Adapter, Index 6 (100% over 36bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTATGC5210.40849929433903087TruSeq Adapter, Index 11 (97% over 35bp)
TGTGCTCTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTA4530.35518268778422457TruSeq Adapter, Index 11 (97% over 37bp)
GAAGAGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAA4300.33714912968480476TruSeq Adapter, Index 11 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTATG4010.31441116512466677TruSeq Adapter, Index 11 (97% over 36bp)
GTGACTGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCT3950.30970675866394853TruSeq Adapter, Index 11 (97% over 37bp)
GACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGAT3750.29402540379488784TruSeq Adapter, Index 11 (97% over 37bp)
AAATTAATACGACTCACTATAGATCGGAAGAGCACACGTCTGAACTCCAG3720.2916732005645288Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGCTCTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAG3660.28696879410381054TruSeq Adapter, Index 11 (97% over 37bp)
TGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAG3300.2587423553395013TruSeq Adapter, Index 11 (97% over 37bp)
TGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGA3130.24541320370079972TruSeq Adapter, Index 11 (97% over 37bp)
CTGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAG3100.24306100047044063TruSeq Adapter, Index 11 (97% over 37bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGT3090.2422769327269876TruSeq Adapter, Index 11 (97% over 37bp)
AATTAATACGACTCACTATAGGGGTAGATCGGAAGAGCACACGTCTGAAC2900.22737964560137994Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGCTCTTCCGATCTGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAG2860.22424337462756783TruSeq Adapter, Index 6 (97% over 36bp)
ACCCCTATAGTGAGTCGTATTAATTAGATCGGAAGAGCACACGTCTGAAC2700.21169829073231927Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCAGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTA2660.20856201975850713TruSeq Adapter, Index 6 (97% over 36bp)
TGTGACTGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC2550.19993727458052377TruSeq Adapter, Index 6 (100% over 36bp)
AAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTATGCCGTC2520.19758507135016465TruSeq Adapter, Index 11 (96% over 31bp)
AGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTATGCCGTCT2450.19209659714599342TruSeq Adapter, Index 11 (96% over 30bp)
CGTGTGCTCTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCT2210.1732789713031206TruSeq Adapter, Index 11 (97% over 37bp)
TGACTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCT2200.17249490355966754TruSeq Adapter, Index 11 (97% over 37bp)
TGTGCTCTTCCGATCTGAAGAGCACACGTCTGAACTCCAGTCACGCCTTA2130.1670064293554963TruSeq Adapter, Index 6 (97% over 36bp)
CTCTTCCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC2080.16308609063823115TruSeq Adapter, Index 6 (100% over 36bp)
GAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGAT2030.15916575192096596TruSeq Adapter, Index 11 (97% over 37bp)
AGATGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTAT1980.1552454132037008TruSeq Adapter, Index 11 (96% over 33bp)
GTACAAAGAATTCCTGCAGAGATCGGAAGAGCACACGTCTGAACTCCAGT1900.14897287125607653Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCT1860.1458366002822644TruSeq Adapter, Index 11 (97% over 37bp)
TGCTCTTCCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1730.13564371961737492Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AATTAATACGACTCACTATAGGGGAGATCGGAAGAGCACACGTCTGAACT1580.12388270346557943Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAA1570.1230986357221264TruSeq Adapter, Index 11 (97% over 37bp)
GAAGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAG1470.11525795828759605TruSeq Adapter, Index 11 (97% over 37bp)
CTCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAA1350.10584914536615964TruSeq Adapter, Index 11 (97% over 37bp)
GAGACGGGAAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCT1350.10584914536615964TruSeq Adapter, Index 11 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTAGATCGGAAGAGCACACGTCTGAA1350.10584914536615964Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GAGATCAGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGT1340.10506507762270662TruSeq Adapter, Index 6 (97% over 36bp)
CTGTGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTA1330.10428100987925357TruSeq Adapter, Index 11 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)