FastQCFastQC Report
Fri 2 Nov 2018
EXB015.A1701_S0_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEXB015.A1701_S0_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127540
Sequences flagged as poor quality0
Sequence length76
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3293125.820134859651873No Hit
GAACTCCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTC78186.129841618315822Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTGTAGATC47463.721185510428101Illumina Single End PCR Primer 1 (96% over 33bp)
CCAGTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCG31292.4533479692645446Illumina Single End PCR Primer 1 (96% over 33bp)
GTGACTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCG20561.6120432805394387Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCCAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACT16261.2748941508546339Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTGT14561.141602634467618Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTG13081.025560608436569Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCCAGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGAC11290.8852124823584757Illumina Single End PCR Primer 1 (96% over 33bp)
GTGACTGGAGTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAG10570.8287596048298573Illumina Single End PCR Primer 1 (96% over 33bp)
CCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTGTA9540.7480006272541947Illumina Single End PCR Primer 1 (96% over 33bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTGTAGAT8990.7048769013642778Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGT7280.570801317233809Illumina Single End PCR Primer 1 (96% over 33bp)
CCAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTGT5900.46259996863729025Illumina Single End PCR Primer 1 (96% over 33bp)
GAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTG5480.4296691234122628Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCCAGTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAG4690.3677277716794731Illumina Single End PCR Primer 1 (96% over 33bp)
GAAGAGCACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACT4120.3230359103026501Illumina Single End PCR Primer 1 (96% over 33bp)
ACGCTCTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTC3930.3081386231770425Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCAGTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGA3570.27991218441273324Illumina Single End PCR Primer 1 (96% over 33bp)
CCAGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTG3500.27442371020856204Illumina Single End PCR Primer 1 (96% over 33bp)
ATAGTGAGTCGTATTAATTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAG3440.2697193037478438Illumina Single End PCR Primer 1 (100% over 30bp)
GAAGAGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCG3380.2650148972871256Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCG3060.23992472949662852Illumina Single End PCR Primer 1 (96% over 33bp)
CCAGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGT3000.2352203230359103Illumina Single End PCR Primer 1 (96% over 33bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTGTAGAT2890.22659557785792692Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCG2860.22424337462756783Illumina Single End PCR Primer 1 (96% over 33bp)
ACCCCTATAGTGAGTCGTATTAATTAGATCGGAAGAGCGTCGTGTAGGGA2690.21091422298886625Illumina Single End PCR Primer 1 (100% over 25bp)
AATTAATACGACTCACTATAGGGGTAGATCGGAAGAGCGTCGTGTAGGGA2530.19836913909361767Illumina Single End PCR Primer 1 (100% over 25bp)
GAACTCAGTCACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAG2210.1732789713031206Illumina Single End PCR Primer 1 (96% over 33bp)
GAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTGTA2000.15681354869060687Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCCAGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGA1980.1552454132037008Illumina Single End PCR Primer 1 (96% over 33bp)
GAAGAGCACACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGA1950.15289320997334171Illumina Single End PCR Primer 1 (96% over 33bp)
CTCTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTG1870.1466206680257174Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCGGAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAG1870.1466206680257174Illumina Single End PCR Primer 1 (96% over 33bp)
CTGCAGGAATTCTTTGTACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT1850.14505253253881137Illumina Single End PCR Primer 1 (100% over 31bp)
GAACTCGGAAGAGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCC1620.12701897443939156Illumina Single End PCR Primer 1 (96% over 33bp)
CCAGTCACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTC1400.10976948408342481Illumina Single End PCR Primer 1 (96% over 33bp)
CCCCTATAGTGAGTCGTATTAATTAGATCGGAAGAGCGTCGTGTAGGGAA1360.10663321310961267Illumina Single End PCR Primer 1 (100% over 26bp)
CGCTCTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCG1340.10506507762270662Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCAGGAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCA1280.1003606711619884Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)