FastQCFastQC Report
Fri 2 Nov 2018
EXB015.A1701_S0_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEXB015.A1701_S0_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122311
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTATGCCGT2066316.893819852670653TruSeq Adapter, Index 11 (96% over 32bp)
CTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAA80816.606928240305451TruSeq Adapter, Index 11 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTA48193.939956340803362TruSeq Adapter, Index 11 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTAT31632.586030692251719TruSeq Adapter, Index 11 (97% over 37bp)
GTGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAG31322.560685465738977TruSeq Adapter, Index 11 (97% over 37bp)
CCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAG21601.7659899763717082TruSeq Adapter, Index 11 (97% over 37bp)
GAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGAT16961.3866291666326005TruSeq Adapter, Index 11 (97% over 37bp)
ACTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTA16481.3473849449354516TruSeq Adapter, Index 11 (97% over 37bp)
GACTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTT14391.1765090629624482TruSeq Adapter, Index 11 (97% over 37bp)
AGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATC14271.166698007538161TruSeq Adapter, Index 11 (97% over 37bp)
CTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCT11790.9639361954362241TruSeq Adapter, Index 11 (97% over 37bp)
GAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC10980.897711571322285TruSeq Adapter, Index 6 (100% over 36bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGT10350.8462035303447768TruSeq Adapter, Index 11 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTATGCC8590.7023080507885635TruSeq Adapter, Index 11 (97% over 34bp)
GGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGA8330.6810507640359411TruSeq Adapter, Index 11 (97% over 37bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTATGCCG5450.4455854338530467TruSeq Adapter, Index 11 (96% over 33bp)
ACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATC5430.4439502579489989TruSeq Adapter, Index 11 (97% over 37bp)
CTCTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGAT5220.4267809109564961TruSeq Adapter, Index 11 (97% over 37bp)
CTCTTCCGATCTGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGAT4910.401435684443754TruSeq Adapter, Index 6 (97% over 36bp)
GTGACTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC4840.39571256877958644TruSeq Adapter, Index 6 (100% over 36bp)
AGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTATGCCGTCT4590.375272869978988TruSeq Adapter, Index 11 (96% over 30bp)
GTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCT4500.3679145784107725TruSeq Adapter, Index 11 (97% over 37bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTATGC4400.35973869889053317TruSeq Adapter, Index 11 (97% over 35bp)
GTGACTGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCT3970.3245824169535038TruSeq Adapter, Index 11 (97% over 37bp)
GAAGAGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAA3860.31558894948124044TruSeq Adapter, Index 11 (97% over 37bp)
TGTGCTCTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTA3760.30741306996100104TruSeq Adapter, Index 11 (97% over 37bp)
GACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGAT3520.2877909591124265TruSeq Adapter, Index 11 (97% over 37bp)
AAATTAATACGACTCACTATAGATCGGAAGAGCACACGTCTGAACTCCAG3410.2787974916401632Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTATG3300.26980402416789984TruSeq Adapter, Index 11 (97% over 36bp)
TGCTCTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAG3100.2534522651274211TruSeq Adapter, Index 11 (97% over 37bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGT3090.25263467717539717TruSeq Adapter, Index 11 (97% over 37bp)
AAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTATGCCGTC3070.2509995012713493TruSeq Adapter, Index 11 (96% over 31bp)
TGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGA2870.23464774223087048TruSeq Adapter, Index 11 (97% over 37bp)
TGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAG2840.2321949783747987TruSeq Adapter, Index 11 (97% over 37bp)
CTGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAG2660.21747839523836776TruSeq Adapter, Index 11 (97% over 37bp)
AATTAATACGACTCACTATAGGGGTAGATCGGAAGAGCACACGTCTGAAC2660.21747839523836776Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTATGCCGTCTT2580.21093769162217627TruSeq Adapter, Index 11 (96% over 29bp)
AGATCAGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTA2410.19703869643776928TruSeq Adapter, Index 6 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2300.18804522896550596No Hit
TGCTCTTCCGATCTGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAG2280.1864100530614581TruSeq Adapter, Index 6 (97% over 36bp)
CTCTTCCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC2250.18395728920538626TruSeq Adapter, Index 6 (100% over 36bp)
GAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGAT2230.18232211330133838TruSeq Adapter, Index 11 (97% over 37bp)
TGTGACTGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC2180.17823417354121868TruSeq Adapter, Index 6 (100% over 36bp)
AGATGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTAT1960.16024723859669202TruSeq Adapter, Index 11 (96% over 33bp)
TGACTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCT1950.1594296506446681TruSeq Adapter, Index 11 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATATCGTATGCCGT1820.14880100726835688TruSeq Adapter, Index 11 (96% over 32bp)
ACCCCTATAGTGAGTCGTATTAATTAGATCGGAAGAGCACACGTCTGAAC1820.14880100726835688Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTACAAAGAATTCCTGCAGAGATCGGAAGAGCACACGTCTGAACTCCAGT1690.1381723638920457Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGTGTGCTCTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCT1610.13163166027585418TruSeq Adapter, Index 11 (97% over 37bp)
TGTGCTCTTCCGATCTGAAGAGCACACGTCTGAACTCCAGTCACGCCTTA1600.13081407232383024TruSeq Adapter, Index 6 (97% over 36bp)
CTCTTCCTGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC1590.12999648437180628TruSeq Adapter, Index 5 (97% over 35bp)
TGTGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAA1570.12836130846775842TruSeq Adapter, Index 11 (97% over 37bp)
TTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTC1510.1234557807556148TruSeq Adapter, Index 11 (97% over 37bp)
AGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTATGCCGTCTTC1500.12263819280359084TruSeq Adapter, Index 11 (96% over 28bp)
GAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGCTCTCGTATGCCGT1370.11200954942727963TruSeq Adapter, Index 11 (96% over 32bp)
TGCTCTTCCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1350.11037437352323176Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGAAGAGCGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1330.10873919761918388Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAAGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAG1320.10792160966715994TruSeq Adapter, Index 11 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTAGATCGGAAGAGCACACGTCTGAA1260.10301608195501633Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)