EXB015.A1701_S0_L003_R2_001.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	EXB015.A1701_S0_L003_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	122311
Sequences flagged as poor quality	0
Sequence length	76
%GC	67

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	30078	24.591410420976036	No Hit
GAACTCCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTC	7208	5.8931739581885525	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTGTAGATC	4774	3.9031648829622845	Illumina Single End PCR Primer 1 (96% over 33bp)
CCAGTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCG	2806	2.2941517933791724	Illumina Single End PCR Primer 1 (96% over 33bp)
GTGACTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCG	2000	1.6351759040478782	Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTG	1594	1.3032351955261587	Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTGT	1460	1.193678409954951	Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCCAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACT	1427	1.166698007538161	Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCCAGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGAC	1329	1.086574388239815	Illumina Single End PCR Primer 1 (96% over 33bp)
CCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTGTA	1199	0.9802879544767029	Illumina Single End PCR Primer 1 (96% over 33bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTGTAGAT	1098	0.897711571322285	Illumina Single End PCR Primer 1 (96% over 33bp)
GTGACTGGAGTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAG	1064	0.869913580953471	Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGT	751	0.6140085519699782	Illumina Single End PCR Primer 1 (96% over 33bp)
CCAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTGT	562	0.4594844290374537	Illumina Single End PCR Primer 1 (96% over 33bp)
GAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTG	498	0.4071588001079216	Illumina Single End PCR Primer 1 (96% over 33bp)
GAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTGTA	480	0.39244221697149073	Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCCAGTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAG	429	0.35074523141826985	Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCAGTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGA	370	0.30250754224885745	Illumina Single End PCR Primer 1 (96% over 33bp)
ACGCTCTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTC	357	0.2918788988725462	Illumina Single End PCR Primer 1 (96% over 33bp)
GAAGAGCACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACT	351	0.2869733711604026	Illumina Single End PCR Primer 1 (96% over 33bp)
ATAGTGAGTCGTATTAATTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAG	325	0.26571608440778016	Illumina Single End PCR Primer 1 (100% over 30bp)
CCAGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTG	304	0.24854673741527747	Illumina Single End PCR Primer 1 (96% over 33bp)
GAAGAGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCG	299	0.24445879765515777	Illumina Single End PCR Primer 1 (96% over 33bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTGTAGAT	281	0.22974221451872684	Illumina Single End PCR Primer 1 (96% over 33bp)
CCAGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGT	273	0.22320151090253534	Illumina Single End PCR Primer 1 (96% over 33bp)
ACCCCTATAGTGAGTCGTATTAATTAGATCGGAAGAGCGTCGTGTAGGGA	253	0.20684975186205656	Illumina Single End PCR Primer 1 (100% over 25bp)
GAACTCCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCG	250	0.20439698800598477	Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCG	243	0.1986738723418172	Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCGGAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAG	215	0.17578140968514688	Illumina Single End PCR Primer 1 (96% over 33bp)
CTCTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTG	212	0.17332864582907506	Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCAGTCACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAG	198	0.16188241450073992	Illumina Single End PCR Primer 1 (96% over 33bp)
GAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTGTAG	186	0.15207135907645264	Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCCAGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGA	182	0.14880100726835688	Illumina Single End PCR Primer 1 (96% over 33bp)
AATTAATACGACTCACTATAGGGGTAGATCGGAAGAGCGTCGTGTAGGGA	174	0.14226030365216538	Illumina Single End PCR Primer 1 (100% over 25bp)
CTGCAGGAATTCTTTGTACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT	172	0.1406251277481175	Illumina Single End PCR Primer 1 (100% over 31bp)
GAAGAGCACACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGA	151	0.1234557807556148	Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCAGGAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCA	146	0.11936784099549509	Illumina Single End PCR Primer 1 (96% over 33bp)
CCAGTCACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTC	140	0.11446231328335146	Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCGGAAGAGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCC	127	0.10383366990704024	Illumina Single End PCR Primer 1 (96% over 33bp)

Adapter Content

Adapter graph

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content