FastQCFastQC Report
Fri 2 Nov 2018
EXB015.A1701_S0_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEXB015.A1701_S0_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108968
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTATGCCGT1888317.328940606416563TruSeq Adapter, Index 11 (96% over 32bp)
CTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAA74046.794655311651127TruSeq Adapter, Index 11 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTA37583.4487188899493426TruSeq Adapter, Index 11 (97% over 37bp)
GTGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAG30092.761361133543793TruSeq Adapter, Index 11 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTAT27492.5227589751119597TruSeq Adapter, Index 11 (97% over 37bp)
CCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAG18761.721606343146612TruSeq Adapter, Index 11 (97% over 37bp)
ACTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTA14531.3334189853902063TruSeq Adapter, Index 11 (97% over 37bp)
AGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATC11431.0489317964907128TruSeq Adapter, Index 11 (97% over 37bp)
GAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGAT9360.8589677703545995TruSeq Adapter, Index 11 (97% over 37bp)
GAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC9100.8351075545114162TruSeq Adapter, Index 6 (100% over 36bp)
GACTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTT8010.7350781880919169TruSeq Adapter, Index 11 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTATGCC7250.6653329417810734TruSeq Adapter, Index 11 (97% over 34bp)
GGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGA6510.5974230966889361TruSeq Adapter, Index 11 (97% over 37bp)
CTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCT6450.5919168930328169TruSeq Adapter, Index 11 (97% over 37bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTATGCCG5460.5010645327068497TruSeq Adapter, Index 11 (96% over 33bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGT5460.5010645327068497TruSeq Adapter, Index 11 (97% over 37bp)
CTCTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGAT4720.43315468761471254TruSeq Adapter, Index 11 (97% over 37bp)
CTCTTCCGATCTGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGAT4680.42948388517729974TruSeq Adapter, Index 6 (97% over 36bp)
ACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATC4330.39736436384993756TruSeq Adapter, Index 11 (97% over 37bp)
GTGACTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC4060.3725864473974011TruSeq Adapter, Index 6 (100% over 36bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTATGC3880.3560678364290434TruSeq Adapter, Index 11 (97% over 35bp)
TGTGCTCTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTA3700.3395492254606857TruSeq Adapter, Index 11 (97% over 37bp)
GAAGAGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAA3450.3166067102268556TruSeq Adapter, Index 11 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTATG3320.30467660230526394TruSeq Adapter, Index 11 (97% over 36bp)
AAATTAATACGACTCACTATAGATCGGAAGAGCACACGTCTGAACTCCAG3160.28999339255561263Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTGACTGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCT3130.287240290727553TruSeq Adapter, Index 11 (97% over 37bp)
TGCTCTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAG3120.2863225901181998TruSeq Adapter, Index 11 (97% over 37bp)
GACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGAT3090.2835694882901402TruSeq Adapter, Index 11 (97% over 37bp)
CTGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAG2810.2578738712282505TruSeq Adapter, Index 11 (97% over 37bp)
TGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAG2730.25053226635342485TruSeq Adapter, Index 11 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2720.24961456574407165No Hit
AATTAATACGACTCACTATAGGGGTAGATCGGAAGAGCACACGTCTGAAC2560.2349313559944204Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGA2430.2230012480728287TruSeq Adapter, Index 11 (97% over 37bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGT2330.21382424197929667TruSeq Adapter, Index 11 (97% over 37bp)
CTCTTCCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC2230.20464723588576464TruSeq Adapter, Index 6 (100% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGCTCTCGTATGCCGT2090.19179942735481978TruSeq Adapter, Index 11 (96% over 32bp)
GAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGAT2010.18445782247999412TruSeq Adapter, Index 11 (97% over 37bp)
TGACTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCT2000.18354012187064092TruSeq Adapter, Index 11 (97% over 37bp)
TGTGACTGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC1900.1743631157771089TruSeq Adapter, Index 6 (100% over 36bp)
TGCTCTTCCGATCTGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAG1890.17344541516775566TruSeq Adapter, Index 6 (97% over 36bp)
ACCCCTATAGTGAGTCGTATTAATTAGATCGGAAGAGCACACGTCTGAAC1810.16610381029293003Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCAGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTA1740.1596799060274576TruSeq Adapter, Index 6 (97% over 36bp)
TGTGCTCTTCCGATCTGAAGAGCACACGTCTGAACTCCAGTCACGCCTTA1710.15692680419939797TruSeq Adapter, Index 6 (97% over 36bp)
GTACAAAGAATTCCTGCAGAGATCGGAAGAGCACACGTCTGAACTCCAGT1670.15325600176198517Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTAT1610.14774979810586594TruSeq Adapter, Index 11 (96% over 33bp)
GAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATATCGTATGCCGT1590.14591439688715954TruSeq Adapter, Index 11 (96% over 32bp)
AGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTATGCCGTCT1530.1404081932310403TruSeq Adapter, Index 11 (96% over 30bp)
CTCTTCCTGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC1390.12756038470009542TruSeq Adapter, Index 5 (97% over 35bp)
GAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGAGCTCGTATGCCGT1330.12205418104397621TruSeq Adapter, Index 11 (96% over 32bp)
CATCTAGTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTA1270.11654797738785698TruSeq Adapter, Index 11 (97% over 37bp)
GAAGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAG1240.11379487555979736TruSeq Adapter, Index 11 (97% over 37bp)
TGTGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAA1240.11379487555979736TruSeq Adapter, Index 11 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATATCGTAT1240.11379487555979736TruSeq Adapter, Index 11 (97% over 37bp)
AAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCTCGTATGCCGTC1190.10920637251303135TruSeq Adapter, Index 11 (96% over 31bp)
CGTGTGCTCTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCT1190.10920637251303135TruSeq Adapter, Index 11 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTAGATCGGAAGAGCACACGTCTGAA1170.10737097129432494Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATATCGTA1130.10370016885691213TruSeq Adapter, Index 11 (97% over 37bp)
TGCTCTTCCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1120.10278246824755892Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTAAGATCT1120.10278246824755892TruSeq Adapter, Index 11 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)