EXB015.A1701_S0_L004_R2_001.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	EXB015.A1701_S0_L004_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	108968
Sequences flagged as poor quality	0
Sequence length	76
%GC	67

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	26341	24.173151750972764	No Hit
GAACTCCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTC	6598	6.054988620512444	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTGTAGATC	3894	3.5735261728213787	Illumina Single End PCR Primer 1 (96% over 33bp)
CCAGTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCG	2682	2.461273034285295	Illumina Single End PCR Primer 1 (96% over 33bp)
GTGACTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCG	1807	1.6582850011012407	Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCCAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACT	1272	1.1673151750972763	Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTGT	1233	1.1315248513325014	Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTG	1025	0.9406431245870347	Illumina Single End PCR Primer 1 (96% over 33bp)
GTGACTGGAGTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAG	903	0.8286836502459437	Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCCAGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGAC	869	0.7974818295279348	Illumina Single End PCR Primer 1 (96% over 33bp)
CCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTGTA	798	0.7323250862638573	Illumina Single End PCR Primer 1 (96% over 33bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTGTAGAT	736	0.6754276484839586	Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGT	634	0.5818221863299318	Illumina Single End PCR Primer 1 (96% over 33bp)
CCAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTGT	461	0.4230599809118273	Illumina Single End PCR Primer 1 (96% over 33bp)
GAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTG	459	0.42122457969312094	Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCCAGTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAG	362	0.33220762058586006	Illumina Single End PCR Primer 1 (96% over 33bp)
GAAGAGCACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACT	343	0.31477130900814915	Illumina Single End PCR Primer 1 (96% over 33bp)
ACGCTCTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTC	309	0.2835694882901402	Illumina Single End PCR Primer 1 (96% over 33bp)
CCAGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTG	301	0.27622788341531457	Illumina Single End PCR Primer 1 (96% over 33bp)
GAAGAGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCG	296	0.27163938036854857	Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCAGTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGA	287	0.2633800748843697	Illumina Single End PCR Primer 1 (96% over 33bp)
ATAGTGAGTCGTATTAATTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAG	283	0.2597092724469569	Illumina Single End PCR Primer 1 (100% over 30bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTGTAGAT	266	0.24410836208795242	Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCG	258	0.23676675721312682	Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCG	250	0.22942515233830116	Illumina Single End PCR Primer 1 (96% over 33bp)
ACCCCTATAGTGAGTCGTATTAATTAGATCGGAAGAGCGTCGTGTAGGGA	236	0.21657734380735627	Illumina Single End PCR Primer 1 (100% over 25bp)
CCAGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGT	228	0.20923573893253064	Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCGGAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAG	205	0.18812862491740695	Illumina Single End PCR Primer 1 (96% over 33bp)
CTCTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTG	178	0.16335070846487043	Illumina Single End PCR Primer 1 (96% over 33bp)
AATTAATACGACTCACTATAGGGGTAGATCGGAAGAGCGTCGTGTAGGGA	177	0.1624330078555172	Illumina Single End PCR Primer 1 (100% over 25bp)
GAACTCCAGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGA	176	0.161515307246164	Illumina Single End PCR Primer 1 (96% over 33bp)
GAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCGTGTA	170	0.15600910359004477	Illumina Single End PCR Primer 1 (96% over 33bp)
GAACTCAGTCACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAG	169	0.15509140298069157	Illumina Single End PCR Primer 1 (96% over 33bp)
CTGCAGGAATTCTTTGTACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT	164	0.15050289993392554	Illumina Single End PCR Primer 1 (100% over 31bp)
GAACTCAGGAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCA	128	0.1174656779972102	Illumina Single End PCR Primer 1 (96% over 33bp)
AGTTCAGACGTGTGCTCTTCCGATCTAGATCGGAAGAGCGTCGTGTAGGG	121	0.11104177373173775	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAAGAGCACACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGA	115	0.10553557007561853	Illumina Single End PCR Primer 1 (96% over 33bp)
CGCTCTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTCG	115	0.10553557007561853	Illumina Single End PCR Primer 1 (96% over 33bp)
GCACTAGATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACT	114	0.10461786946626532	Illumina Single End PCR Primer 1 (96% over 33bp)
CCAGTCACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAGACTC	111	0.1018647676382057	Illumina Single End PCR Primer 1 (96% over 33bp)

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content