nextflow run nf-core/eager -r dev \ --pairedEnd \ --reads 'data/Sample1/Sample1.pass_{1,2}.fastq.gz' \ --fasta '/home/keaton/Reference/EAGER/rCRS/rCRS.fasta' \ --bwa_index '/home/keaton/Reference/EAGER/rCRS/rCRS.fasta' \ --fasta_index '/home/keaton/Reference/EAGER/rCRS/rCRS.fasta.fai' \ --outdir output/ \ --clip_readlength 35 \ --clip_min_read_quality 0 \ --clip_min_adap_overlap 3 \ --bwaalnn 0.01 \ --bwaalnl 16500 \ --bam_mapping_quality_threshold 30 \ --max_cpus 10 \ --max_memory 64.G \ --max_time 8.h \ -name 'DedupTest_Run1'