Input data to the Zika pipeline arrives in the data/ directory. This should be mounted to data/ in the Docker container.
datausvi-library1-2016-12-10- libraryraw_reads- squiggle graphs in fast5 formatpass- contains.fast5files
basecalled_reads- basecalled with Metrichorpass_demultiplex- demultiplexed basecalled readsNB01- contains.fast5files for NB01 barcodeNB02- contains.fast5files for NB02 barcode- etc...
nonNB_demultiplexed- demultiplexed basecalled readsBC01- contains.fast5files for BC01 barcodeBC02- contains.fast5files for BC02 barcode- etc...
fail- contains.fast5files that weren't demultiplexed
Sample metadata for the Zika pipeline arrives in the samples/ directory. This should be mounted to samples/ in the Docker container.
samples/samples.tsv- line list of sample metadataruns.tsv- line list of run metadata
Must be tsv formatted. Keyed off of column headers rather than column order.
| sample_id | strain | collection_date | country | division | location |
|---|---|---|---|---|---|
| ZBRD116 | ZBRD116 | 2015-08-28 | brazil | alagoas | arapiraca |
| ZBRC301 | ZBRC301 | 2015-05-13 | brazil | pernambuco | paulista |
Must be tsv formatted. Keyed off of column headers rather than column order.
| run_name | barcode_id | sample_id | primer_scheme |
|---|---|---|---|
| library1 | NB01 | ZBRD116 | v2_500.amplicons.ver2 |