I tried to correct a single sequence using proovread. During the estimation steps for masked positions I received the following errors/warnings:
Use of uninitialized value $bpN in division (/) at ../../proovread/bin/proovread line 1716, <$xgr> line 1.
Use of uninitialized value $bpN in division (/) at ../../proovread/bin/proovread line 1718, <$xgr> line 1.
Obviously, this resulted in
Masked : 0.0%
Masked < 92% total / > 3% new - continuing
Therefore, no correction step will be skipped even if everything was already masked.
Further investigation of that problem point to the SeqFilter script, which
"[..]might be ommited if to few sequences are retrieved[...]"
In my case, the N90 value was omitted and therefore, the value for masked positions is undefined.
I will provide a fix for that issue
I tried to correct a single sequence using proovread. During the estimation steps for masked positions I received the following errors/warnings:
Obviously, this resulted in
Therefore, no correction step will be skipped even if everything was already masked.
Further investigation of that problem point to the
SeqFilterscript, whichIn my case, the N90 value was omitted and therefore, the value for masked positions is undefined.
I will provide a fix for that issue