I was wondering why some sequence results are completely excluded after running Method A (vote method) as opposed to Method D (besthit method)? I have 25 sequences in my BLAST input file and yet only 18 of them appear in the result tsv file after running Method A. In the result tsv file after running Method D, all 25 of them are present. I would appreciate it very much if you could let me know the reason for this discrepancy? For example why are Zotu1 & Zotu2 sequence results missing from my vote method output tsv file but not from the besthit output tsv file? I'm attaching here all the input and output files for your kind perusal.
Below are the command-lines that I had used
blastMining besthit -i newforBLASTmining_10_test_zotus_coverage_blastn_maxtarget10_screenresults.txt -o new_besthit_method -e 0.001 -pi 97 -n 10 -sm test_zotus -j 100 -p besthit_method -kp
blastMining vote -i newforBLASTmining_10_test_zotus_coverage_blastn_maxtarget10_screenresults.txt -o vote_method -e 0.001 -txl 99,97,95,90,85,80,75 -n 10 -sm test_zotus -j 100 -p vote_method -kp
I was wondering why some sequence results are completely excluded after running Method A (vote method) as opposed to Method D (besthit method)? I have 25 sequences in my BLAST input file and yet only 18 of them appear in the result tsv file after running Method A. In the result tsv file after running Method D, all 25 of them are present. I would appreciate it very much if you could let me know the reason for this discrepancy? For example why are Zotu1 & Zotu2 sequence results missing from my vote method output tsv file but not from the besthit output tsv file? I'm attaching here all the input and output files for your kind perusal.
Below are the command-lines that I had used
newforBLASTmining_10_test_zotus_coverage_blastn_maxtarget10_screenresults.txt
besthit_method.tsv.txt
vote_method.tsv.txt
Here's the sequences fasta file that I had used to perform the BLASTn analysis
test_select_zotus.fasta.txt