|
587 | 587 | "bt2n": { |
588 | 588 | "type": "integer", |
589 | 589 | "description": "Specify the -N parameter for bowtie2 (mismatches in seed). This will override defaults from alignmode/sensitivity.", |
590 | | - "default": "0", |
| 590 | + "default": 0, |
591 | 591 | "fa_icon": "fas fa-sort-numeric-down", |
592 | | - "help_text": "The number of mismatches allowed in the seed during seed-and-extend procedure of Bowtie2. This will override any values set with `--bt2_sensitivity`. Can either be 0 or 1. Default: 0 (i.e. use`--bt2_sensitivity` defaults).\n\n> Modifies Bowtie2 parameters: `-N`" |
| 592 | + "help_text": "The number of mismatches allowed in the seed during seed-and-extend procedure of Bowtie2. This will override any values set with `--bt2_sensitivity`. Can either be 0 or 1. Default: 0 (i.e. use`--bt2_sensitivity` defaults).\n\n> Modifies Bowtie2 parameters: `-N`", |
| 593 | + "enum": [ |
| 594 | + 0, |
| 595 | + 1 |
| 596 | + ] |
593 | 597 | }, |
594 | 598 | "bt2l": { |
595 | 599 | "type": "integer", |
| 600 | + "default": 0, |
596 | 601 | "description": "Specify the -L parameter for bowtie2 (length of seed substrings). This will override defaults from alignmode/sensitivity.", |
597 | 602 | "fa_icon": "fas fa-ruler-horizontal", |
598 | | - "default": "0", |
599 | 603 | "help_text": "The length of the seed sub-string to use during seeding. This will override any values set with `--bt2_sensitivity`. Default: 0 (i.e. use`--bt2_sensitivity` defaults: [20 for local and 22 for end-to-end](http://bowtie-bio.sourceforge.net/bowtie2/manual.shtml#command-line).\n\n> Modifies Bowtie2 parameters: `-L`" |
600 | 604 | }, |
601 | 605 | "bt2_trim5": { |
602 | 606 | "type": "integer", |
| 607 | + "default": 0, |
603 | 608 | "description": "Specify number of bases to trim off from 5' (left) end of read before alignment.", |
604 | 609 | "fa_icon": "fas fa-cut", |
605 | | - "default": "0", |
606 | 610 | "help_text": "Number of bases to trim at the 5' (left) end of read prior alignment. Maybe useful when left-over sequencing artefacts of in-line barcodes present Default: 0\n\n> Modifies Bowtie2 parameters: `-bt2_trim5`" |
607 | 611 | }, |
608 | 612 | "bt2_trim3": { |
609 | 613 | "type": "integer", |
| 614 | + "default": 0, |
610 | 615 | "description": "Specify number of bases to trim off from 3' (right) end of read before alignment.", |
611 | 616 | "fa_icon": "fas fa-cut", |
612 | | - "default": "0", |
613 | 617 | "help_text": "Number of bases to trim at the 3' (right) end of read prior alignment. Maybe useful when left-over sequencing artefacts of in-line barcodes present Default: 0.\n\n> Modifies Bowtie2 parameters: `-bt2_trim3`" |
614 | 618 | } |
615 | 619 | }, |
|
658 | 662 | "bam_mapping_quality_threshold": { |
659 | 663 | "type": "integer", |
660 | 664 | "description": "Minimum mapping quality for reads filter.", |
661 | | - "default": "0", |
| 665 | + "default": 0, |
662 | 666 | "fa_icon": "fas fa-greater-than-equal", |
663 | 667 | "help_text": "Specify a mapping quality threshold for mapped reads to be kept for downstream analysis. By default keeps all reads and is therefore set to `0` (basically doesn't filter anything).\n\n> Modifies samtools view parameter: `-q`" |
664 | 668 | }, |
665 | 669 | "bam_filter_minreadlength": { |
666 | 670 | "type": "integer", |
| 671 | + "default": 0, |
667 | 672 | "fa_icon": "fas fa-ruler-horizontal", |
668 | 673 | "description": "Specify minimum read length to be kept after mapping.", |
669 | | - "default": "0", |
670 | 674 | "help_text": "Specify minimum length of mapped reads. This filtering will apply at the same time as mapping quality filtering.\n\nIf used _instead_ of minimum length read filtering at AdapterRemoval, this can be useful to get more realistic endogenous DNA percentages, when most of your reads are very short (e.g. in single-stranded libraries) and would otherwise be discarded by AdapterRemoval (thus making an artificially small denominator for a typical endogenous DNA calculation). Note in this context you should not perform mapping quality filtering nor discarding of unmapped reads to ensure a correct denominator of all reads, for the endogenous DNA calculation.\n\n> Modifies filter_bam_fragment_length.py parameter: `-l`" |
671 | 675 | }, |
672 | 676 | "bam_unmapped_type": { |
|
851 | 855 | }, |
852 | 856 | "bamutils_clip_half_udg_left": { |
853 | 857 | "type": "integer", |
854 | | - "default": "1", |
| 858 | + "default": 1, |
855 | 859 | "fa_icon": "fas fa-ruler-combined", |
856 | 860 | "description": "Specify the number of bases to clip off reads from 'left' end of read for half-UDG libraries.", |
857 | 861 | "help_text": "Default set to `1` and clips off one base of the left or right side of reads from libraries whose UDG treatment is set to `half`. Note that reverse reads will automatically be clipped off at the reverse side with this (automatically reverses left and right for the reverse read).\n\n> Modifies bamUtil's trimBam parameter: `-L -R`" |
858 | 862 | }, |
859 | 863 | "bamutils_clip_half_udg_right": { |
860 | 864 | "type": "integer", |
861 | | - "default": "1", |
| 865 | + "default": 1, |
862 | 866 | "fa_icon": "fas fa-ruler", |
863 | 867 | "description": "Specify the number of bases to clip off reads from 'right' end of read for half-UDG libraries.", |
864 | 868 | "help_text": "Default set to `1` and clips off one base of the left or right side of reads from libraries whose UDG treatment is set to `half`. Note that reverse reads will automatically be clipped off at the reverse side with this (automatically reverses left and right for the reverse read).\n\n> Modifies bamUtil's trimBam parameter: `-L -R`" |
865 | 869 | }, |
866 | 870 | "bamutils_clip_none_udg_left": { |
867 | 871 | "type": "integer", |
868 | | - "default": "1", |
| 872 | + "default": 1, |
869 | 873 | "fa_icon": "fas fa-ruler-combined", |
870 | 874 | "description": "Specify the number of bases to clip off reads from 'left' end of read for non-UDG libraries.", |
871 | 875 | "help_text": "Default set to `1` and clips off one base of the left or right side of reads from libraries whose UDG treatment is set to `none`. Note that reverse reads will automatically be clipped off at the reverse side with this (automatically reverses left and right for the reverse read).\n\n> Modifies bamUtil's trimBam parameter: `-L -R`" |
872 | 876 | }, |
873 | 877 | "bamutils_clip_none_udg_right": { |
874 | 878 | "type": "integer", |
875 | | - "default": "1", |
| 879 | + "default": 1, |
876 | 880 | "fa_icon": "fas fa-ruler", |
877 | 881 | "description": "Specify the number of bases to clip off reads from 'right' end of read for non-UDG libraries.", |
878 | 882 | "help_text": "Default set to `1` and clips off one base of the left or right side of reads from libraries whose UDG treatment is set to `none`. Note that reverse reads will automatically be clipped off at the reverse side with this (automatically reverses left and right for the reverse read).\n\n> Modifies bamUtil's trimBam parameter: `-L -R`" |
|
1023 | 1027 | }, |
1024 | 1028 | "freebayes_g": { |
1025 | 1029 | "type": "integer", |
| 1030 | + "default": 0, |
1026 | 1031 | "description": "Specify to skip over regions of high depth by discarding alignments overlapping positions where total read depth is greater than specified in --freebayes_C.", |
1027 | 1032 | "fa_icon": "fab fa-think-peaks", |
1028 | | - "default": "0", |
1029 | 1033 | "help_text": "Specify to skip over regions of high depth by discarding alignments overlapping positions where total read depth is greater than specified C. Not set by default.\n\n> Modifies freebayes parameter: `-g`" |
1030 | 1034 | }, |
1031 | 1035 | "freebayes_p": { |
|
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