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@@ -45,20 +45,28 @@ Additional functionality contained by the pipeline currently includes:
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## Quick Start
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1. Install [`nextflow`](docs/installation.md)
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2. Install one of [`docker`](https://docs.docker.com/engine/installation/), [`singularity`](https://www.sylabs.io/guides/3.0/user-guide/) or [`conda`](https://conda.io/miniconda.html)
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3. Download the EAGER pipeline
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```bash
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nextflow pull nf-core/eager
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```
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4. Set up your job with default parameters
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4. Test the pipeline using the provided test data
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```bash
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nextflow run nf-core/eager -profile <docker/singularity/conda>,test --pairedEnd
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```
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5. Start running your own ancient DNA analysis!
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```bash
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nextflow run nf-core -profile <docker/singularity/conda> --reads'*_R{1,2}.fastq.gz' --fasta '<REFERENCE>.fasta'
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nextflow run nf-core/eager -profile <docker/singularity/conda> --reads'*_R{1,2}.fastq.gz' --fasta '<REFERENCE>.fasta'
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```
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5. See the overview of the run with under`<OUTPUT_DIR>/MultiQC/multiqc_report.html`
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NB. You can see an overview of the run in the MultiQC report located at`<OUTPUT_DIR>/MultiQC/multiqc_report.html`
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Modifications to the default pipeline are easily made using various options
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