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Using GATKs mergeVcfs#571

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FriederikeHanssen merged 26 commits intonf-core:devfrom
asp8200:merge_vcfs
Jun 3, 2022
Merged

Using GATKs mergeVcfs#571
FriederikeHanssen merged 26 commits intonf-core:devfrom
asp8200:merge_vcfs

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@asp8200 asp8200 commented May 31, 2022

Closes #569

Using GATK4's module mergeVcfs instead of modules/local/concat_vcf/main.nf + bin/concatenateVCFs.sh.

With this PR, the nf-core-module with GATK4's mergeVcfs is now being included in Sarek.

  • This comment contains a description of changes (with reason).
  • If you've fixed a bug or added code that should be tested, add tests!
    • If you've added a new tool - add to the software_versions process and a regex to scrape_software_versions.py
    • If you've added a new tool - have you followed the pipeline conventions in the contribution docs
    • If necessary, also make a PR on the nf-core/sarek branch on the nf-core/test-datasets repository.
  • Make sure your code lints (nf-core lint).
  • Ensure the test suite passes (nextflow run . -profile test,docker).
  • Usage Documentation in docs/usage.md is updated.
  • Output Documentation in docs/output.md is updated.
  • CHANGELOG.md is updated.
  • README.md is updated (including new tool citations and authors/contributors).

@asp8200 asp8200 requested a review from FriederikeHanssen May 31, 2022 16:25
Comment thread conf/modules.config
Comment thread modules.json
Comment thread subworkflows/nf-core/variantcalling/freebayes/main.nf Outdated
@maxulysse
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<3

@asp8200 asp8200 marked this pull request as ready for review June 2, 2022 12:44
@asp8200 asp8200 requested a review from maxulysse as a code owner June 2, 2022 12:44
@maxulysse
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some failures in the CI.
I don't care much about gatk4_spark and mutect2 as I know they're difficult, but concerned about haplotypecaller and variantcalling_channel

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Great work. Just update the changelog and two indents and then ready to merge 🚀

Edit: Didn't see the failing variantcaller test. Let me see if I can find a reason quickly

Comment thread subworkflows/nf-core/variantcalling/manta/germline/main.nf Outdated
Comment thread subworkflows/nf-core/variantcalling/manta/somatic/main.nf Outdated
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asp8200 commented Jun 2, 2022

some failures in the CI. I don't care much about gatk4_spark and mutect2 as I know they're difficult, but concerned about haplotypecaller and variantcalling_channel

@FriederikeHanssen confirmed that there is no problem with the variantcalling_channel. It was apparently just a glitch in the execution of the CI-tests on GitHub. (There seems to be a lot of such glitches with the CI-tests recently.)

The problem with the haplotypecaller-test is that the test expects an output-vcf-file called sample1.vcf.gz but the hyplotypecaller produces a vcf-file called sample1.g.vcf.gz. The problem is also on the dev-branch (6cf0b12). The docs/output.md seem to indicate that the output-vcf-files should be named in another way:

sarek/docs/output.md

Lines 229 to 232 in c60ef1f

**Output directory: `results/VariantCalling/[SAMPLE]/HaplotypeCallerGVCF`**
- `HaplotypeCaller_[SAMPLE].g.vcf.gz` and `HaplotypeCaller_[SAMPLE].g.vcf.gz.tbi`
- `VCF` with Tabix index

Comment thread CHANGELOG.md Outdated
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@nf-core-bot fix linting please

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Yeah don't worry about haplotypecaller, the PR is already open to fix that subworkflow, just need to finish it. I will fix the CI there

@FriederikeHanssen FriederikeHanssen self-requested a review June 3, 2022 08:19
@FriederikeHanssen FriederikeHanssen merged commit f84f5c6 into nf-core:dev Jun 3, 2022
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3 participants