Skip to content

Process rnaseq rsem/salmon output, accept additional species from igenomes#118

Merged
WackerO merged 64 commits intoqbic-pipelines:devfrom
WackerO:newseq2
May 11, 2022
Merged

Process rnaseq rsem/salmon output, accept additional species from igenomes#118
WackerO merged 64 commits intoqbic-pipelines:devfrom
WackerO:newseq2

Conversation

@WackerO
Copy link
Copy Markdown
Contributor

@WackerO WackerO commented Apr 21, 2022

Many thanks to contributing to qbic-pipelines!

This PR allows rnadeseq to use the rsem/salmon output from the new rnaseq version. Further, it contains igenomes.config so that additional species can be used; it should also be able to handle species not configured by igenomes as long as the user provides the necessary files/params.

PR checklist

  • This comment contains a description of changes (with reason)
  • If you've fixed a bug or added code that should be tested, add tests!
  • Ensure the test suite passes (nextflow run . -profile test,docker).
  • Documentation in docs is updated
  • CHANGELOG.md is updated
  • README.md is updated

Learn more about contributing: https://github.com/qbic-pipelines/rnadeseq/tree/master/.github/CONTRIBUTING.md

@WackerO WackerO changed the title Newseq2 Process rnaseq rsem/salmon output, accept additional species from igenomes Apr 21, 2022
Copy link
Copy Markdown
Collaborator

@ggabernet ggabernet left a comment

Choose a reason for hiding this comment

The reason will be displayed to describe this comment to others. Learn more.

Hi @WackerO, great job! I just had some comments and reached until the DESeq2 script for reviewing

Comment thread modules/local/deseq2.nf Outdated
Comment thread bin/DESeq2.R Outdated
Comment thread bin/DESeq2.R Outdated
Comment thread bin/DESeq2.R Outdated
Comment thread bin/DESeq2.R Outdated
Comment thread bin/DESeq2.R Outdated
Comment thread bin/DESeq2.R
Comment thread bin/DESeq2.R
Comment thread bin/pathway_analysis.R Outdated
Comment thread bin/pathway_analysis.R
WackerO and others added 2 commits May 4, 2022 12:36
Comment thread bin/DESeq2.R Outdated
Copy link
Copy Markdown
Collaborator

@ggabernet ggabernet left a comment

Choose a reason for hiding this comment

The reason will be displayed to describe this comment to others. Learn more.

Hi @WackerO, great job, getting there. Just had some other comments!

Comment thread bin/DESeq2.R
Comment thread bin/pathway_analysis.R Outdated
Comment thread docs/usage.md Outdated
Comment thread docs/usage.md
Comment thread docs/usage.md Outdated
Comment thread nextflow_schema.json Outdated
Comment thread nextflow.config
Comment thread testdata/QDESQ/QDESQ_Sample_preparations.tsv Outdated
Comment thread workflows/rnadeseq.nf Outdated
Comment thread main.nf
WackerO and others added 8 commits May 9, 2022 11:51
Co-authored-by: Gisela Gabernet <gisela.gabernet@gmail.com>
Co-authored-by: Gisela Gabernet <gisela.gabernet@gmail.com>
Co-authored-by: Gisela Gabernet <gisela.gabernet@gmail.com>
Co-authored-by: Gisela Gabernet <gisela.gabernet@gmail.com>
Co-authored-by: Gisela Gabernet <gisela.gabernet@gmail.com>
Copy link
Copy Markdown
Collaborator

@ggabernet ggabernet left a comment

Choose a reason for hiding this comment

The reason will be displayed to describe this comment to others. Learn more.

One more round, but this should be the last one! :)
There are still multiple places where ~~~ is substituted back to ===, which will cause issues in file diffs during merge conflicts. Could you change all back to ~~~

Comment thread conf/base.config Outdated
Comment thread nextflow.config
Comment thread nextflow_schema.json Outdated
Comment thread testdata/QDESQ/QDESQ_Sample_preparations.tsv Outdated
Comment thread main.nf
Comment thread nextflow_schema.json Outdated
Copy link
Copy Markdown
Collaborator

@ggabernet ggabernet left a comment

Choose a reason for hiding this comment

The reason will be displayed to describe this comment to others. Learn more.

Looks good to me!

@WackerO WackerO merged commit abc0b1f into qbic-pipelines:dev May 11, 2022
@WackerO WackerO deleted the newseq2 branch July 27, 2022 09:00
Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment

Labels

None yet

Projects

None yet

Development

Successfully merging this pull request may close these issues.

2 participants