-
Notifications
You must be signed in to change notification settings - Fork 21
Very quick start
Ryan Wick edited this page Jul 31, 2025
·
2 revisions
This page shows how to run Autocycler with the absolute minimum of setup and explanation.
You'll need to install Autocycler and several long-read assemblers. You can use this environment file to put everything in one conda environment.
You can use this Bash script to run the full Autocycler pipeline in one command:
autocycler_full.sh reads.fastq.gz 16 4
Where reads.fastq.gz is your input long-read file, 16 is the number of threads to use and 4 is the number of parallel jobs (for running multiple assemblers at once).
When the script is complete, your final assembly will be here: autocycler_out/consensus_assembly.fasta.
- Step 1: Autocycler subsample
- Step 2: Generating input assemblies
- Step 3: Autocycler compress
- Step 4: Autocycler cluster
- Step 5: Autocycler trim
- Step 6: Autocycler resolve
- Step 7: Autocycler combine