Describe the bug
When running with multiple samples, after Deduplication, DamageProfiler and Qualimap are only run on for a single sample (note that I skipped preseq)
To Reproduce
nextflow run nf-core/eager \
-c /projects1/users/fellows/nextflow/nextflow_custom.config \
-profile shh_custom \
--reads '/projects1/microbiome_calculus/RIII/03-preprocessing/pairment_splitting/raw_se/*/*_R1_*.fastq.gz' \
--singleEnd \
--fasta '/projects1/microbiome_calculus/RIII/01-data/reference_genomes/HG19/hg19_complete.fasta' \
--bwa_index '/projects1/microbiome_calculus/RIII/01-data/reference_genomes/HG19/' \
--seq_dict '/projects1/microbiome_calculus/RIII/01-data/reference_genomes/HG19/hg19_complete.dict' \
--outdir '/projects1/microbiome_calculus/RIII/03-preprocessing/sequencing_qc/raw_se/' \
--name 'RIII_pe_raw' \
--email fellows@shh.mpg.de \
--max_cpus 8 \
--max_mem '64.GB' \
--skip_preseq \
--min_adap_overlap 1 \
--clip_readlength 30 \
--clip_min_read_quality 20 \
--bwaalnn 0.01 \
--bwaalnl 32 \
--bam_discard_unmapped \
--bam_unmapped_type fastq \
--dedupper dedup \
--dedup_all_merged \
-resume \
-r 2.0.5
Expected behavior
Files for all samples generated in DeDup to be run through Qualimap/DamageProfiler
Screenshots
You can see here under DamageProfiler only a single sample, despite in DeDuplication all four samples are procssed
.
├── damageprofiler
│ └── SPYNEW_S0_L001_R1_001.fastq.merged.combined.sorted
│ ├── 3p_freq_misincorporations.txt
│ ├── 3pGtoA_freq.txt
│ ├── 5pCtoT_freq.txt
│ ├── 5p_freq_misincorporations.txt
│ ├── DamagePlot.pdf
│ ├── DamageProfiler.log
│ ├── dmgprof.json
│ ├── DNA_comp_genome.txt
│ ├── DNA_composition_sample.txt
│ ├── identity_histogram.pdf
│ ├── Length_plot.pdf
│ ├── lgdistribution.txt
│ └── misincorporation.txt
├── deduplication
│ └── dedup
│ ├── dedup.log -> /projects1/microbiome_calculus/RIII/03-preprocessing/sequencing_qc/preprocessed_se/work/c2/f7c8c24f2d73efe4a1abbd977f448a/dedup.log
│ ├── ELSIDRON1_S0_L001_R1_001.fastq.merged.combined.sorted.bam.filtered.hist -> /projects1/microbiome_calculus/RIII/03-preprocessing/sequencing_qc/preprocessed_se/work/c2/f7c8c24f2d73efe4a1abbd977f448a/ELSIDRON1_S0_L001_R1_001.fastq.merged.combined.sorted.bam.filtered.hist
│ ├── ELSIDRON1_S0_L001_R1_001.fastq.merged.combined.sorted.bam.filtered.sorted.bam -> /projects1/microbiome_calculus/RIII/03-preprocessing/sequencing_qc/preprocessed_se/work/c2/f7c8c24f2d73efe4a1abbd977f448a/ELSIDRON1_S0_L001_R1_001.fastq.merged.combined.sorted.bam.filtered.sorted.bam
│ ├── ELSIDRON1_S0_L001_R1_001.fastq.merged.combined.sorted.bam.filtered.sorted.bam.bai -> /projects1/microbiome_calculus/RIII/03-preprocessing/sequencing_qc/preprocessed_se/work/c2/f7c8c24f2d73efe4a1abbd977f448a/ELSIDRON1_S0_L001_R1_001.fastq.merged.combined.sorted.bam.filtered.sorted.bam.bai
│ ├── ELSIDRON2_S0_L001_R1_001.fastq.merged.combined.sorted.bam.filtered.hist -> /projects1/microbiome_calculus/RIII/03-preprocessing/sequencing_qc/preprocessed_se/work/e6/b735964cec5775d49d35d5a267c1a8/ELSIDRON2_S0_L001_R1_001.fastq.merged.combined.sorted.bam.filtered.hist
│ ├── ELSIDRON2_S0_L001_R1_001.fastq.merged.combined.sorted.bam.filtered.sorted.bam -> /projects1/microbiome_calculus/RIII/03-preprocessing/sequencing_qc/preprocessed_se/work/e6/b735964cec5775d49d35d5a267c1a8/ELSIDRON2_S0_L001_R1_001.fastq.merged.combined.sorted.bam.filtered.sorted.bam
│ ├── ELSIDRON2_S0_L001_R1_001.fastq.merged.combined.sorted.bam.filtered.sorted.bam.bai -> /projects1/microbiome_calculus/RIII/03-preprocessing/sequencing_qc/preprocessed_se/work/e6/b735964cec5775d49d35d5a267c1a8/ELSIDRON2_S0_L001_R1_001.fastq.merged.combined.sorted.bam.filtered.sorted.bam.bai
│ ├── SPYNEW_S0_L001_R1_001.fastq.merged.combined.sorted.bam.filtered.hist -> /projects1/microbiome_calculus/RIII/03-preprocessing/sequencing_qc/preprocessed_se/work/a7/cb2f1f9edbf7f4b6f9b49fa4cf543d/SPYNEW_S0_L001_R1_001.fastq.merged.combined.sorted.bam.filtered.hist
│ ├── SPYNEW_S0_L001_R1_001.fastq.merged.combined.sorted.bam.filtered.sorted.bam -> /projects1/microbiome_calculus/RIII/03-preprocessing/sequencing_qc/preprocessed_se/work/a7/cb2f1f9edbf7f4b6f9b49fa4cf543d/SPYNEW_S0_L001_R1_001.fastq.merged.combined.sorted.bam.filtered.sorted.bam
│ ├── SPYNEW_S0_L001_R1_001.fastq.merged.combined.sorted.bam.filtered.sorted.bam.bai -> /projects1/microbiome_calculus/RIII/03-preprocessing/sequencing_qc/preprocessed_se/work/a7/cb2f1f9edbf7f4b6f9b49fa4cf543d/SPYNEW_S0_L001_R1_001.fastq.merged.combined.sorted.bam.filtered.sorted.bam.bai
│ ├── SPYOLD_S0_L001_R1_001.fastq.merged.combined.sorted.bam.filtered.hist -> /projects1/microbiome_calculus/RIII/03-preprocessing/sequencing_qc/preprocessed_se/work/1c/07ac33303da3549468f844b3708142/SPYOLD_S0_L001_R1_001.fastq.merged.combined.sorted.bam.filtered.hist
│ ├── SPYOLD_S0_L001_R1_001.fastq.merged.combined.sorted.bam.filtered.sorted.bam -> /projects1/microbiome_calculus/RIII/03-preprocessing/sequencing_qc/preprocessed_se/work/1c/07ac33303da3549468f844b3708142/SPYOLD_S0_L001_R1_001.fastq.merged.combined.sorted.bam.filtered.sorted.bam
│ └── SPYOLD_S0_L001_R1_001.fastq.merged.combined.sorted.bam.filtered.sorted.bam.bai -> /projects1/microbiome_calculus/RIII/03-preprocessing/sequencing_qc/preprocessed_se/work/1c/07ac33303da3549468f844b3708142/SPYOLD_S0_L001_R1_001.fastq.merged.combined.sorted.bam.filtered.sorted.bam.bai
Additional context
Please provide me with the following files:
nextflow.log
eager_report.html.txt
eager_timeline.html.txt
eager_trace.txt
Describe the bug
When running with multiple samples, after Deduplication, DamageProfiler and Qualimap are only run on for a single sample (note that I skipped preseq)
To Reproduce
Expected behavior
Files for all samples generated in DeDup to be run through Qualimap/DamageProfiler
Screenshots
You can see here under DamageProfiler only a single sample, despite in DeDuplication all four samples are procssed
Additional context
Please provide me with the following files:
nextflow.log
eager_report.html.txt
eager_timeline.html.txt
eager_trace.txt